Bulboacă Adriana Elena, Porfire Alina Silvia, Rus Vasile, Nicula Cristina Ariadna, Bulboacă Corneliu Angelo, Bolboacă Sorana D
Department of Pathophysiology, Iuliu Hațieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Antioxidants (Basel). 2022 Feb 17;11(2):412. doi: 10.3390/antiox11020412.
Our study aimed to assess the effect of liposomal epigallocatechin-gallate (LEGCG) compared with epigallocatechin-gallate (EGCG) solution on hepatic toxicity induced by gentamicin (G) administration in rats. Five groups were evaluated, a control group (no G administration) and four groups that received G (1 mL, i.p, 80 mg/kg b.w. (body weight/day), for 7 days) to which we associated daily administration 30 min before G of EGCG (G-EGCG, 2.5 mg/0.1 kg b.w.), LEGCG (G-LEGCG, 2.5 mg/0.1 kg b.w.) or silymarin (100 mg/kg b.w./day). The nitro-oxidative stress (NOx), catalase (CAT), TNF-α, transaminases, creatinine, urea, metalloproteinase (MMP) 2 and 9, and liver histopathological changes were evaluated. LEGCG exhibited better efficacy than EGCG, improving the oxidant/antioxidant balance ( = 0.0125 for NOx and 0.0032 for CAT), TNF-α ( < 0.0001), MMP-2 ( < 0.0001), aminotransferases ( = 0.0001 for AST and 0.0136 for ALT), creatinine ( < 0.0001), urea ( = 0.0006) and histopathologic liver changes induced by gentamicin. Our study demonstrated the beneficial effect of EGCG with superior results of the liposomal formulation for hepatoprotection in experimental hepatic toxicity induced by gentamicin.
我们的研究旨在评估与表没食子儿茶素没食子酸酯（EGCG）溶液相比，脂质体表没食子儿茶素没食子酸酯（LEGCG）对庆大霉素（G）诱导的大鼠肝毒性的影响。评估了五组，一组为对照组（未给予G），另外四组给予G（1 mL，腹腔注射，80 mg/kg体重/天，共7天），并在每天给予G前30分钟分别联合给予EGCG（G-EGCG，2.5 mg/0.1 kg体重）、LEGCG（G-LEGCG，2.5 mg/0.1 kg体重）或水飞蓟宾（100 mg/kg体重/天）。评估了硝基氧化应激（NOx）、过氧化氢酶（CAT）、肿瘤坏死因子-α（TNF-α）、转氨酶、肌酐、尿素、金属蛋白酶（MMP）2和9以及肝脏组织病理学变化。LEGCG表现出比EGCG更好的疗效，改善了氧化/抗氧化平衡（NOx为0.0125，CAT为0.0032）、TNF-α（<0.0001）、MMP-2（<0.0001）、氨基转移酶（天冬氨酸转氨酶为0.0001，丙氨酸转氨酶为0.0136）、肌酐（<0.0001）、尿素（0.0006）以及庆大霉素诱导的肝脏组织病理学变化。我们的研究证明了EGCG的有益作用，脂质体制剂在庆大霉素诱导的实验性肝毒性中具有更好的肝脏保护效果。
