• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

LPS 给药通过选择性剪接影响神经胶质免疫程序。

LPS Administration Impacts Glial Immune Programs by Alternative Splicing.

机构信息

Laboratory of Functional Neurogenomics, Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia.

出版信息

Biomolecules. 2022 Feb 8;12(2):277. doi: 10.3390/biom12020277.

DOI:10.3390/biom12020277
PMID:35204777
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8961644/
Abstract

We performed transcriptome analysis in the hippocampus 24 h after lipopolysaccharide (LPS) administration. We observed glial-specific genes, comprised of two-thirds of all differentially expressed genes (DEGs). We found microglial DEGs that were the most numerous in LPS group. On the contrary, differential alternative splicing (DAS) analysis revealed the most numerous DAS events in astrocytes. Besides, we observed distinct major isoform switching in the gene, with skipping of exon 8 in LPS group. usually considered a pluripotency sustaining agent in brain embryonic development, according to the previous studies. Analyzing the splicing tune-up upon LPS exposure, we came to a supposition that the short isoform de-represses immune-specific response by adjusted splicing architecture. Additionally, the () immune-specific splicing factor has apparently been de-repressed by the short isoform in glial cells. Notably, both the and genes express primarily in microglial/endothelial brain cells. We also report immune-related genes, altering their major isoforms upon LPS exposure. The results revealed immune modulating role of alternative splicing in brain.

摘要

我们在脂多糖（LPS）给药后 24 小时对海马体进行了转录组分析。我们观察到了胶质细胞特异性基因，占所有差异表达基因（DEG）的三分之二。我们发现 LPS 组中小胶质细胞的 DEG 数量最多。相反，差异剪接（DAS）分析显示星形胶质细胞中 DAS 事件数量最多。此外，我们观察到 基因的主要同工型转换明显，LPS 组中第 8 个外显子缺失。根据先前的研究，通常被认为是大脑胚胎发育中维持多能性的物质。分析 LPS 暴露后的剪接调整，我们推测短的 同工型通过调整剪接结构来解除对免疫特异性反应的抑制。此外，在神经胶质细胞中，免疫特异性剪接因子 （） 明显被短的 同工型解除抑制。值得注意的是，和 基因主要在小胶质细胞/内皮脑细胞中表达。我们还报告了免疫相关基因，它们在 LPS 暴露后改变其主要同工型。结果揭示了大脑中剪接的免疫调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1b058eb99743/biomolecules-12-00277-g018.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/a62e69787484/biomolecules-12-00277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/bd0e4d7dbe75/biomolecules-12-00277-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/7f8fb697d7d4/biomolecules-12-00277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1f1696f50864/biomolecules-12-00277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/f3ca7fe0a597/biomolecules-12-00277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1c1301584f80/biomolecules-12-00277-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/ab9e861efd32/biomolecules-12-00277-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/6762defa139d/biomolecules-12-00277-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/cea09b0c36b3/biomolecules-12-00277-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/0d89b128682a/biomolecules-12-00277-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/4912102102b3/biomolecules-12-00277-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/8e8d781f4c3d/biomolecules-12-00277-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/d0408d7a93f4/biomolecules-12-00277-g013a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/ebbaf844745f/biomolecules-12-00277-g014a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/2efd6122ff7d/biomolecules-12-00277-g015a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/7dcf8f219386/biomolecules-12-00277-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/d61a96a95545/biomolecules-12-00277-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1b058eb99743/biomolecules-12-00277-g018.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/a62e69787484/biomolecules-12-00277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/bd0e4d7dbe75/biomolecules-12-00277-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/7f8fb697d7d4/biomolecules-12-00277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1f1696f50864/biomolecules-12-00277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/f3ca7fe0a597/biomolecules-12-00277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1c1301584f80/biomolecules-12-00277-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/ab9e861efd32/biomolecules-12-00277-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/6762defa139d/biomolecules-12-00277-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/cea09b0c36b3/biomolecules-12-00277-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/0d89b128682a/biomolecules-12-00277-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/4912102102b3/biomolecules-12-00277-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/8e8d781f4c3d/biomolecules-12-00277-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/d0408d7a93f4/biomolecules-12-00277-g013a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/ebbaf844745f/biomolecules-12-00277-g014a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/2efd6122ff7d/biomolecules-12-00277-g015a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/7dcf8f219386/biomolecules-12-00277-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/d61a96a95545/biomolecules-12-00277-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23e9/8961644/1b058eb99743/biomolecules-12-00277-g018.jpg

相似文献

1
LPS Administration Impacts Glial Immune Programs by Alternative Splicing.LPS 给药通过选择性剪接影响神经胶质免疫程序。
Biomolecules. 2022 Feb 8;12(2):277. doi: 10.3390/biom12020277.
2
Skipping of exon 10 in pre-mRNA regulated by PTBP1 mediates invasion and metastasis process of liver cancer cells.PTBP1 调控的前 mRNA 外显子 10 跳跃促进肝癌细胞的侵袭和转移过程。
Theranostics. 2020 Apr 27;10(13):5719-5735. doi: 10.7150/thno.42010. eCollection 2020.
3
PTBP1-dependent regulation of USP5 alternative RNA splicing plays a role in glioblastoma tumorigenesis.PTBP1 依赖性调节 USP5 可变剪接在胶质母细胞瘤发生中起作用。
Mol Carcinog. 2012 Nov;51(11):895-906. doi: 10.1002/mc.20859. Epub 2011 Oct 4.
4
Identification and Characterization of a Minimal Functional Splicing Regulatory Protein, PTBP1.鉴定和表征最小功能剪接调控蛋白 PTBP1。
Biochemistry. 2020 Dec 22;59(50):4766-4774. doi: 10.1021/acs.biochem.0c00664. Epub 2020 Dec 7.
5
Aberrant Exon 8/8a Splicing by Downregulated PTBP (Polypyrimidine Tract-Binding Protein) 1 Increases Ca1.2 Dihydropyridine Resistance to Attenuate Vasodilation.下调的 PTBP1（多嘧啶 tract 结合蛋白 1）通过异常的外显子 8/8a 剪接增加 Ca1.2 二氢吡啶耐药性，从而减弱血管舒张。
Arterioscler Thromb Vasc Biol. 2020 Oct;40(10):2440-2453. doi: 10.1161/ATVBAHA.120.315010. Epub 2020 Aug 13.
6
High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin.PTBP1的高表达通过对皮层肌动蛋白的可变剪接促进结直肠癌的侵袭。
Oncotarget. 2017 May 30;8(22):36185-36202. doi: 10.18632/oncotarget.15873.
7
The latest role of nerve-specific splicing factor PTBP1 in the transdifferentiation of glial cells into neurons.神经特异性剪接因子PTBP1在胶质细胞向神经元转分化中的最新作用。
Wiley Interdiscip Rev RNA. 2023 Mar;14(2):e1740. doi: 10.1002/wrna.1740. Epub 2022 May 16.
8
Neural Isoforms of Agrin Are Generated by Reduced PTBP1-RNA Interaction Network Spanning the Neuron-Specific Splicing Regions in .神经特异性剪接区跨越 Agrin 的 PTBP1-RNA 相互作用网络减少导致 Agrin 的神经同型物的产生。
Int J Mol Sci. 2023 Apr 18;24(8):7420. doi: 10.3390/ijms24087420.
9
PTBP1 enhances exon11a skipping in Mena pre-mRNA to promote migration and invasion in lung carcinoma cells.PTBP1 通过增强 Mena 前体 mRNA 外显子 11a 的剪接促进肺癌细胞的迁移和侵袭。
Biochim Biophys Acta Gene Regul Mech. 2019 Aug;1862(8):858-869. doi: 10.1016/j.bbagrm.2019.04.006. Epub 2019 May 7.
10
Alternative splicing regulation of membrane trafficking genes during myogenesis.在肌发生过程中，膜转运基因的可变剪接调控。
RNA. 2022 Apr;28(4):523-540. doi: 10.1261/rna.078993.121. Epub 2022 Jan 26.

引用本文的文献

1
Brainstem transcriptomic changes in male Wistar rats after acute stress, comparing the use of duplex specific nuclease (DSN).急性应激后雄性 Wistar 大鼠脑干转录组变化，比较使用双链特异性核酸酶 (DSN)。
Sci Rep. 2024 Sep 19;14(1):21856. doi: 10.1038/s41598-024-73042-2.
2
YTHDC1 promotes the malignant progression of gastric cancer by promoting ROD1 translocation to the nucleus.YTHDC1 通过促进 ROD1 向核内易位促进胃癌的恶性进展。
Cell Biol Toxicol. 2024 Apr 4;40(1):19. doi: 10.1007/s10565-024-09859-4.
3
Immune and metabolic challenges induce changes in pain sensation and related pathways in the hypothalamus.

本文引用的文献

1
Identifying the Involvement of Pro-Inflammatory Signal in Hippocampal Gene Expression Changes after Experimental Ischemia: Transcriptome-Wide Analysis.确定促炎信号在实验性缺血后海马基因表达变化中的作用：全转录组分析
Biomedicines. 2021 Dec 5;9(12):1840. doi: 10.3390/biomedicines9121840.
2
Nonsense-mediated mRNA decay uses complementary mechanisms to suppress mRNA and protein accumulation.无意义介导的 mRNA 降解利用互补机制来抑制 mRNA 和蛋白质的积累。
Life Sci Alliance. 2021 Dec 8;5(3). doi: 10.26508/lsa.202101217. Print 2022 Mar.
3
SON drives oncogenic RNA splicing in glioblastoma by regulating PTBP1/PTBP2 switching and RBFOX2 activity.
免疫和代谢挑战会引起下丘脑疼痛感知和相关通路的变化。
Physiol Genomics. 2024 Apr 1;56(4):343-359. doi: 10.1152/physiolgenomics.00134.2023. Epub 2024 Jan 8.
4
Inhibition of NO Production in LPS-Stimulated Primary Rat Glial Cells by Gnidilatimonoein and Extract of .狼毒大戟单萜醇和提取物对脂多糖刺激的原代大鼠胶质细胞中一氧化氮产生的抑制作用 。 你提供的原文似乎不完整，最后的“Extract of.”后面缺少具体内容。
Iran J Biotechnol. 2023 Apr 1;21(2):e3052. doi: 10.30498/ijb.2023.285965.3052. eCollection 2023 Apr.
SON 通过调控 PTBP1/PTBP2 转换和 RBFOX2 活性来驱动神经胶质瘤中的致癌 RNA 剪接。
Nat Commun. 2021 Sep 21;12(1):5551. doi: 10.1038/s41467-021-25892-x.
4
Highly accurate protein structure prediction with AlphaFold.利用 AlphaFold 进行高精度蛋白质结构预测。
Nature. 2021 Aug;596(7873):583-589. doi: 10.1038/s41586-021-03819-2. Epub 2021 Jul 15.
5
Alternative splicing during mammalian organ development.哺乳动物器官发育过程中的可变剪接。
Nat Genet. 2021 Jun;53(6):925-934. doi: 10.1038/s41588-021-00851-w. Epub 2021 May 3.
6
An extended catalogue of tandem alternative splice sites in human tissue transcriptomes.人类组织转录组中串联替代剪接位点的扩展目录。
PLoS Comput Biol. 2021 Apr 7;17(4):e1008329. doi: 10.1371/journal.pcbi.1008329. eCollection 2021 Apr.
7
PTBP1 is necessary for dendritic cells to regulate T-cell homeostasis and antitumour immunity.PTBP1 对于树突状细胞调节 T 细胞动态平衡和抗肿瘤免疫是必需的。
Immunology. 2021 May;163(1):74-85. doi: 10.1111/imm.13304. Epub 2021 Feb 3.
8
Genes associated with cognitive performance in the Morris water maze: an RNA-seq study.与 Morris 水迷宫认知表现相关的基因：一项 RNA-seq 研究。
Sci Rep. 2020 Dec 16;10(1):22078. doi: 10.1038/s41598-020-78997-6.
9
Platelets as Mediators of Neuroinflammation and Thrombosis.血小板作为神经炎症和血栓形成的介质
Front Immunol. 2020 Oct 6;11:548631. doi: 10.3389/fimmu.2020.548631. eCollection 2020.
10
Alternative splicing of neurexins 1-3 is modulated by neuroinflammation in the prefrontal cortex of a murine model of multiple sclerosis.神经连接蛋白 1-3 的可变剪接受多发性硬化症小鼠模型前额叶神经炎症调节。
Exp Neurol. 2021 Jan;335:113497. doi: 10.1016/j.expneurol.2020.113497. Epub 2020 Oct 12.