Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Division of Periodontology and Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Biomolecules. 2022 Feb 12;12(2):304. doi: 10.3390/biom12020304.
Periodontal ligament (PDL) cells play a pivotal role in periodontal and bone homeostasis and have promising potential for regenerative medicine and tissue engineering. There is compelling evidence that long non-coding RNAs (lncRNAs) are differentially expressed in PDL cells compared to other cell types and that these lncRNAs are involved in a variety of biological processes. This study systematically reviews the current evidence regarding the expression and regulatory functions of lncRNAs in PDL cells during various biological processes. A systematic search was conducted on PubMed, the Web of Science, Embase, and Google Scholar to include articles published up to 1 July 2021. Original research articles that investigated the expression or regulation of lncRNAs in PDL cells were selected and evaluated for a systematic review. Fifty studies were ultimately included, based on our eligibility criteria. Thirteen of these studies broadly explored the expression profiles of lncRNAs in PDL cells using microarray or RNA sequencing. Nineteen studies investigated the mechanisms by which lncRNAs regulate osteogenic differentiation in PDL cells. The remaining 18 studies investigated the mechanism by which lncRNAs regulate the responses of PDL cells to various stimuli, namely, lipopolysaccharide-induced inflammation, tumor necrosis factor alpha-induced inflammation, mechanical stress, oxidative stress, or hypoxia. We systematically reviewed studies on the expression and regulatory roles of lncRNAs in diverse biological processes in PDL cells, including osteogenic differentiation and cellular responses to inflammation, mechanical stress, and other stimuli. These results provide new insights that may guide the development of lncRNA-based therapeutics for periodontal and bone regeneration.
牙周膜（PDL）细胞在牙周和骨稳态中发挥着关键作用，并且在再生医学和组织工程方面具有广阔的应用前景。有强有力的证据表明，长链非编码 RNA（lncRNA）在 PDL 细胞中的表达与其他细胞类型存在差异，并且这些 lncRNA 参与了多种生物学过程。本研究系统综述了目前关于 lncRNA 在 PDL 细胞在各种生物学过程中的表达和调控功能的研究证据。我们在 PubMed、Web of Science、Embase 和 Google Scholar 上进行了系统检索，纳入截至 2021 年 7 月 1 日发表的文章。选择并评估了研究 PDL 细胞中 lncRNA 表达或调控的原始研究文章，以进行系统综述。根据我们的入选标准，最终有 50 项研究符合条件。其中 13 项研究广泛使用微阵列或 RNA 测序技术来探索 PDL 细胞中 lncRNA 的表达谱。19 项研究调查了 lncRNA 调节 PDL 细胞成骨分化的机制。其余 18 项研究调查了 lncRNA 调节 PDL 细胞对各种刺激（如脂多糖诱导的炎症、肿瘤坏死因子-α诱导的炎症、机械应力、氧化应激或低氧）反应的机制。我们系统地综述了 lncRNA 在 PDL 细胞中在多种生物学过程中的表达和调控作用的研究，包括成骨分化和细胞对炎症、机械应激和其他刺激的反应。这些结果为基于 lncRNA 的牙周和骨再生治疗方法的开发提供了新的思路。
