Hektoen Martin M, Willassen Endre, Budaeva Nataliya
Department of Natural History, NTNU University Museum, Norwegian University of Science and Technology, 7491 Trondheim, Norway.
Åkerblå AS, Nordfrøyveien 413, 7260 Sistranda, Norway.
Biology (Basel). 2022 Feb 18;11(2):327. doi: 10.3390/biology11020327.
Audouin & Milne-Edwards, 1833 is a species rich genus that is common in tropical and subtropical regions. The genus is readily identified by its striking, spiral branchiae, but species identification has historically been challenging due to a high variation in diagnostic characters used. This study aims to reconstruct the phylogeny of with molecular markers and assess the species diversity of West African with the species delimitation programs bPTP and BPP. Specimens were collected from Morocco to Angola, and the markers COI, 16S and 28S were sequenced from 76 specimens. The constructed phylogeny retrieved as monophyletic, as well as five well supported clades within the genus. All clades were defined by morphological characters, some of which have previously not been considered to have high phylogenetic or taxonomical value. Species delimitation analyses recovered 17 new species, several of which were not readily identified morphologically. One species complex comprising between one and 12 species was left unresolved due to incongruence between the species delimitation methods and challenging morphology. Our results indicate that the diversity of is significantly underestimated, where this regional study near to doubled the number ofknown species from the East Atlantic.
奥杜安氏属（Audouin & Milne-Edwards，1833）是一个物种丰富的属，在热带和亚热带地区很常见。该属很容易通过其显著的螺旋鳃来识别，但由于所使用的诊断特征变化很大，历史上物种鉴定一直具有挑战性。本研究旨在利用分子标记重建该属的系统发育，并使用物种界定程序bPTP和BPP评估西非该属的物种多样性。标本采集自摩洛哥至安哥拉，对76个标本的细胞色素氧化酶亚基I（COI）、16S和28S标记进行了测序。构建的系统发育树显示该属为单系类群，以及该属内五个得到充分支持的分支。所有分支都由形态特征定义，其中一些特征以前未被认为具有高系统发育或分类学价值。物种界定分析发现了17个新物种，其中一些在形态上不易识别。由于物种界定方法与具有挑战性的形态之间存在不一致，一个包含1至12个物种的物种复合体仍未得到解决。我们的结果表明，该属的多样性被显著低估，在这项区域研究中，东大西洋已知物种数量几乎翻倍。
