Papp A C, Crowe L, Pettigrew L C, Wu K K
Thromb Res. 1986 May 15;42(4):549-56. doi: 10.1016/0049-3848(86)90218-5.
Production of eicosanoids by deendothelialized aorta in response to continuous infusions of arachidonic acid and platelet suspensions was determined in a rabbit aorta perfusion model. 6-keto-PGF1 alpha production was stimulated by AA infusion in a dose-related manner. Infusion of AA at 4 micrograms/ml/min led to an initial production rate of 0.64 +/- 0.29 ng/min which gradually increased to 0.93 +/- 0.11 ng/min at the 20th min of infusion. When the concentration of AA infusion was increased to 10 micrograms/ml/min, 6-keto-PGF1 alpha production increased to 1.14 +/- 0.86 ng/min initially but declined with time. PGE2 production in response to AA 10 micrograms/min/ml was steady at around 5 ng/min while PGF2 alpha and TXB2 production were only slightly above the control. Perfusion of rabbit washed platelet suspensions at a rate of 3 X 10(8) plt/ml/min raised 6KPGF1 alpha production. The production was further increased when platelets were pretreated with 1-benzylimidazole (5 mM), along with a concurrent reduction in TXB2 release. Pretreatment of platelets with aspirin, on the other hand, abolished the increase in 6KPGF1 alpha production. Our data indicated that the vascular smooth muscle cells can efficiently utilize PGH2 produced by platelets to synthesize PGI2.
在兔主动脉灌注模型中，测定去内皮主动脉对连续输注花生四烯酸和血小板悬液产生类二十烷酸的情况。6-酮-前列腺素F1α的产生以剂量相关方式受到花生四烯酸输注的刺激。以4微克/毫升/分钟的速度输注花生四烯酸，导致初始产生速率为0.64±0.29纳克/分钟，在输注第20分钟时逐渐增加到0.93±0.11纳克/分钟。当花生四烯酸输注浓度增加到10微克/毫升/分钟时，6-酮-前列腺素F1α的产生最初增加到1.14±0.86纳克/分钟，但随时间下降。对10微克/分钟/毫升花生四烯酸的反应中，前列腺素E2的产生稳定在约5纳克/分钟，而前列腺素F2α和血栓素B2的产生仅略高于对照。以3×10⁸血小板/毫升/分钟的速度灌注兔洗涤血小板悬液可提高6-酮-前列腺素F1α的产生。当血小板用1-苄基咪唑（5毫摩尔）预处理时，产生进一步增加，同时血栓素B2释放减少。另一方面，用阿司匹林预处理血小板消除了6-酮-前列腺素F1α产生的增加。我们的数据表明，血管平滑肌细胞可以有效地利用血小板产生的前列腺素H2来合成前列环素。
