School of Kinesiology and Health Science, Muscle Health Research Centre (MHRC), York University, Toronto, ON, Canada.
Methods Mol Biol. 2022;2459:73-78. doi: 10.1007/978-1-0716-2144-8_7.
Apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) is an adaptor protein that is essential for the activation of several inflammasome complexes. Activation of inflammasomes leads to pathogenic clearance and inflammatory cell death called pyroptosis. Upon inflammasome activation, ASC oligomerization leads to the recruitment and activation of caspase-1, which in turn converts pro-inflammatory cytokines (e.g., pro-IL-1β, pro-IL-18) to their mature active form. Given its central role in inflammasome activation, ASC oligomerization is used as an indicator of inflammasome activation. Here we describe how ASC oligomerization can be detected by Western blotting.
凋亡相关斑点样蛋白含有半胱氨酸蛋白酶募集结构域（ASC），是一种衔接蛋白，对于几种炎性小体复合物的激活至关重要。炎性小体的激活导致病原体清除和炎症细胞死亡，称为细胞焦亡。炎性小体激活后，ASC 寡聚化导致半胱氨酸蛋白酶-1的募集和激活，反过来将促炎细胞因子（例如，前白细胞介素-1β，前白细胞介素-18）转化为其成熟的活性形式。鉴于其在炎性小体激活中的核心作用，ASC 寡聚化被用作炎性小体激活的指标。在这里，我们描述了如何通过 Western blot 检测 ASC 寡聚化。
