全血中 NLRP3、AIM2 和 NLRC4 炎性小体的活性和表达分析。
Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood.
机构信息
Department of Pediatrics, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
Department of Pediatrics, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
出版信息
Methods Mol Biol. 2022;2459:105-116. doi: 10.1007/978-1-0716-2144-8_11.
Pro-inflammatory caspase-1 is a key player in innate immunity. Following activation in heterogenic protein complexes called the inflammasome , caspase-1 processes IL-1β and IL-18 to their mature forms and triggers pyroptosis. Here, we describe a small-volume whole blood assay facilitating the measurement of caspase-1 activity and inflammasome-related gene expression following specific stimulation of either the NLRP3, NLRC4, or AIM2 inflammasome .
促炎型半胱天冬酶-1 是先天免疫中的关键分子。在被称为炎性体的异源蛋白复合物激活后，半胱天冬酶-1 将 IL-1β 和 IL-18 加工成成熟形式，并引发细胞焦亡。在此，我们描述了一种小体积全血检测方法，可在特异性刺激 NLRP3、NLRC4 或 AIM2 炎性体后，用于测量半胱天冬酶-1 活性和炎性体相关基因表达。
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