Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA, USA.
Alliance Pharma Inc, Malvern, PA, USA.
Methods Mol Biol. 2022;2455:49-62. doi: 10.1007/978-1-0716-2128-8_5.
Fatty acid beta oxidation (FAO) is a predominant bioenergetic pathway in mammals. Substantial investigations have demonstrated that FAO activity is dysregulated in many pathophysiological conditions including nonalcoholic steatohepatitis (NASH). Convenient and quantitative assays of FAO activities are important for studies of cell metabolism and the biological relevance of FAO to health and diseases. However, most current FAO assays are based on non-physiological culture conditions, measure FAO activity indirectly or lack adequate quantification. We herein describe details of practical protocols for measurement of basal and genetically or pharmacologically regulated FAO activities in the mammalian system. We also discuss the advantages and disadvantages of these assays in the context of experimental purposes.
脂肪酸β氧化（FAO）是哺乳动物的主要生物能量途径。大量研究表明，FAO 活性在许多病理生理条件下失调，包括非酒精性脂肪性肝炎（NASH）。FAO 活性的方便和定量测定对于细胞代谢以及 FAO 对健康和疾病的生物学相关性的研究非常重要。然而，目前大多数 FAO 测定方法基于非生理培养条件，间接测量 FAO 活性或缺乏充分的定量。本文描述了在哺乳动物系统中测量基础和遗传或药理学调节的 FAO 活性的实用方案的详细信息。我们还讨论了这些测定方法在实验目的方面的优缺点。
