Khlebnikova Darya A, Efanova Evgeniya M, Danilova Nina A, Shcherbakova Yaroslava V, Rivera Sidorova Irina
Department of Biotechnology, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Timiryazevskaya 49, 127434 Moscow, Russia.
Plants (Basel). 2022 Feb 16;11(4):533. doi: 10.3390/plants11040533.
Summer savory ( L.) is a medicinal and aromatic plant of the family, a source of valuable secondary metabolites (monoterpenoids, rosmarinic acid, flavonoids). For this paper, flavonoid accumulation in an aseptic culture of summer savory was determined by using a colorimetric method. The organ specificity of flavonoid accumulation in aseptic plants was revealed: In leaves (8.35 ± 0.17 mg/g FW), flower buds (7.55 ± 0.29 mg/g FW), and calyx (5.27 ± 0.28 mg/g FW), flavonoids accumulated in significantly higher amounts than in stems (1.50 ± 0.22 mg/g FW) and corolla (0.78 ± 0.12 mg/g FW). We found that primary callus tissue formed from cotyledon and hypocotyl explants retains the ability to synthesize flavonoids at deficient levels (0.50 ± 0.09 mg/g FW and 0.44 ± 0.11 mg/g FW, respectively), that remained stable throughout six subcultures. Placing the callus tissue in monochrome lighting conditions with blue, green, and red light-emitting diode (LED) lamps leads to morphological changes in the tissue and decreased flavonoid accumulation compared to fluorescent lamps.
夏香薄荷（L.）是唇形科的一种药用和芳香植物，是有价值的次生代谢产物（单萜类化合物、迷迭香酸、黄酮类化合物）的来源。在本文中，采用比色法测定了夏香薄荷无菌培养物中黄酮类化合物的积累。揭示了无菌植物中黄酮类化合物积累的器官特异性：在叶片（8.35±0.17毫克/克鲜重）、花芽（7.55±0.29毫克/克鲜重）和花萼（5.27±0.28毫克/克鲜重）中，黄酮类化合物的积累量显著高于茎（1.50±0.22毫克/克鲜重）和花冠（0.78±0.12毫克/克鲜重）。我们发现，由子叶和下胚轴外植体形成的初代愈伤组织保留了合成黄酮类化合物的能力，但水平较低（分别为0.50±0.09毫克/克鲜重和0.44±0.11毫克/克鲜重），在六次继代培养过程中保持稳定。与荧光灯相比，将愈伤组织置于蓝色、绿色和红色发光二极管（LED）灯的单色光照条件下会导致组织形态发生变化，并降低黄酮类化合物的积累。
