Rippe Karsten, Papantonis Argyris
Division of Chromatin Networks, German Cancer Research Center (DKFZ) and Bioquant, Heidelberg, Germany.
Translational Epigenetics Group, Institute of Pathology, University Medical Center Göttingen, Göttingen, Germany.
Curr Opin Cell Biol. 2022 Feb;74:88-96. doi: 10.1016/j.ceb.2022.01.005. Epub 2022 Feb 22.
Distinct clusters of RNA polymerase II are responsible for gene transcription inside eukaryotic cell nuclei. Despite the functional implications of such subnuclear organization, the attributes of these clusters and the mechanisms underlying their formation remain only partially understood. Recently, the concept of proteins and RNA phase-separating into liquid-like droplets was proposed to drive the formation of transcriptionally-active subcompartments. Here, we attempt to reconcile previous with more recent findings, and discuss how the different ways of assembling the active RNA polymerase II transcriptional machinery relate to nuclear compartmentalization.
不同的RNA聚合酶II簇负责真核细胞核内的基因转录。尽管这种核内亚结构具有功能上的意义，但这些簇的特性及其形成机制仍仅被部分理解。最近，有人提出蛋白质和RNA相分离形成液滴，以驱动转录活性亚区室的形成。在这里，我们试图将先前的发现与最新的发现相协调，并讨论组装活性RNA聚合酶II转录机制的不同方式与核区室化之间的关系。
