Molecular Plant Science/Plant Biochemistry, University of Wuppertal, Gaußstr. 20, 42119, Wuppertal, Germany.
J Plant Physiol. 2022 Apr;271:153645. doi: 10.1016/j.jplph.2022.153645. Epub 2022 Feb 18.
Phloem plays a central role in assimilate transport as well as in the transport of several secondary compounds. In order to study the chemical composition of phloem sap, different methods have been used for its collection, including stem incisions, EDTA-facilitated exudation or aphid stylectomy. Each collection method has several advantages and disadvantages and, unfortunately, the reported metabolite profiles and concentrations depend on the method used for exudate collection. This review therefore primarily focusses on sugars, amino acids, inorganic ions and further transported compounds like organic acids, nucleotides, phytohormons, defense signals, and lipophilic substances in the phloem sap obtained by aphid stylectomy to facilitate comparability of the data.
韧皮部在同化物运输以及几种次生化合物的运输中起着核心作用。为了研究韧皮部汁液的化学成分，已经使用了不同的方法来收集它，包括茎切口、EDTA 促进的渗出或蚜虫刺吸。每种收集方法都有其优缺点，不幸的是，报告的代谢物谱和浓度取决于渗出物收集所用的方法。因此，本综述主要集中在蚜虫刺吸法获得的韧皮部汁液中的糖、氨基酸、无机离子和其他运输化合物，如有机酸、核苷酸、植物激素、防御信号和疏水性物质，以促进数据的可比性。
