Asadollahi Hamed, Vaez Torshizi Rasoul, Ehsani Alireza, Masoudi Ali Akbar
Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
J Anim Breed Genet. 2022 Sep;139(5):574-582. doi: 10.1111/jbg.12675. Epub 2022 Feb 26.
Heat stress is a serious problem in the poultry industry. An effective tool for improving heat tolerance can be genomic selection based on single nucleotide polymorphisms. This study was performed to identify genomic regions controlling survivability to heat stress in a population of F chickens that accidentally experienced acute heat stress, using Illumina 60K Chicken SNP Bead Chip. After quality control in markers, 47,730 SNPs remained for genome-wide association study (GWAS). The GWAS results indicated that markers Gga_rs16111480 (p = 8.503e-08), GGaluGA354375 (p = 5.99e-07) and Gga_rs14748694 (p = 7.085e-07) located on Z chromosome showed significant association with heat stress tolerance trait. The Gga_rs16111480 marker was located inside the CEP78 gene. The marker GGaluGA354375 was located inside the LOC101752071 gene and next to the MEF2C gene. The Gga_rs14748694 marker was adjacent to LOC101752071 and MEF2C genes. Moreover, the SNP maker of Gga_rs16111480 was located on 243 kb downstream of the VPS13A gene, and the GGaluGA354375 and Gga_rs14748694 SNPs were located on 947 kb and 888 kb downstream of the ARRDC3 gene, respectively. The results of this study suggest that apart from the gene LOC101752071, which its function was unknown, each of the two MEF2C and CEP78 genes were found to be closely related to heat stress resistance in bird.
热应激是家禽养殖业中的一个严重问题。基于单核苷酸多态性的基因组选择可能是提高耐热性的一种有效工具。本研究旨在利用Illumina 60K鸡SNP芯片，在一群意外经历急性热应激的F鸡群体中，鉴定控制热应激生存能力的基因组区域。在对标记进行质量控制后，剩余47,730个单核苷酸多态性用于全基因组关联研究（GWAS）。GWAS结果表明，位于Z染色体上的标记Gga_rs16111480（p = 8.503e-08）、GGaluGA354375（p = 5.99e-07）和Gga_rs14748694（p = 7.085e-07）与热应激耐受性状显著相关。标记Gga_rs16111480位于CEP78基因内部。标记GGaluGA354375位于LOC101752071基因内部且靠近MEF2C基因。标记Gga_rs14748694与LOC101752071和MEF2C基因相邻。此外，Gga_rs16111480的单核苷酸多态性标记位于VPS13A基因下游243 kb处，GGaluGA354375和Gga_rs14748694的单核苷酸多态性分别位于ARRDC3基因下游947 kb和888 kb处。本研究结果表明，除了功能未知的LOC101752071基因外，发现MEF2C和CEP78这两个基因中的每一个都与鸟类的热应激抗性密切相关。
