Hoskins S G, Homberg U, Kingan T G, Christensen T A, Hildebrand J G
Cell Tissue Res. 1986;244(2):243-52. doi: 10.1007/BF00219199.
We have prepared and characterized specific rabbit antisera against gamma-aminobutyric acid (GABA) coupled covalently to bovine serum albumin and keyhole-limpet hemocyanin. Using these antisera in immunocytochemical staining procedures, we have probed the antennal lobes and their afferent and efferent fiber tracts in the sphinx moth Manduca sexta for GABA-like immunoreactivity in order to map putatively GABAergic central neurons in the central antennal-sensory pathway. About 30% of the neuronal somata in the large lateral group of cell bodies in the antennal lobe are GABA-immunoreactive; cells in the medial and anterior groups of antennal-lobe cells did not exhibit GABA-like immunoreactivity. GABA-immunoreactive neurites had arborizations in all of the glomeruli in the antennal lobe. Double-labeling experiments involving tandem intracellular staining with Lucifer Yellow and immunocytochemical staining for GABA-like immunoreactivity demonstrated that at least some of the GABA-immunoreactive cells in the antennal lobe are amacrine local interneurons. Several fiber tracts that carry axons of antennal-lobe projection neurons exhibited GABA-immunoreactive fibers. Among the possibly GABA-containing projection neurons are several cells, with somata in the lateral group of the antennal lobe, that send their axons directly to the lateral protocerebrum.
我们制备并鉴定了针对与牛血清白蛋白和钥孔戚血蓝蛋白共价偶联的γ-氨基丁酸（GABA）的特异性兔抗血清。在免疫细胞化学染色程序中使用这些抗血清，我们探测了烟草天蛾触角叶及其传入和传出纤维束中是否存在GABA样免疫反应性，以便绘制触角中央感觉通路中假定的GABA能中枢神经元图谱。触角叶中大型外侧细胞群中约30%的神经元胞体具有GABA免疫反应性；触角叶内侧和前部细胞群中的细胞未表现出GABA样免疫反应性。GABA免疫反应性神经突在触角叶的所有小球中都有分支。涉及用荧光黄进行串联细胞内染色和GABA样免疫反应性免疫细胞化学染色的双标记实验表明，触角叶中至少一些具有GABA免疫反应性的细胞是无长突局部中间神经元。几条携带触角叶投射神经元轴突的纤维束表现出GABA免疫反应性纤维。在可能含有GABA的投射神经元中，有几个细胞的胞体位于触角叶外侧群，它们将轴突直接发送到外侧原脑。
