Meis J F, Rijntjes P J, Verhave J P, Ponnudurai T, Hollingdale M R, Smith J E, Sinden R E, Jap P H, Meuwissen J H, Yap S H
Cell Tissue Res. 1986;244(2):345-50. doi: 10.1007/BF00219210.
Recent advances in the ability to culture the hepatic forms of mammalian malaria parasites, particularly of the important human pathogen Plasmodium falciparum have provided novel opportunities to study the ultrastructural organisation of the parasite in its natural host cell the human hepatocyte. In this electron-microscopic and immunofluorescence study we have found the morphology of both parasite and host cell to be well preserved. The exoerythrocytic forms, which may be found at densities of up to 100/cm2, grow at rates comparable to that in vivo in the chimpanzee. In the multiplying 5- and 7-day schizogonic forms of the ultrastructural organisation of the parasite bears striking resemblances to other mammalian parasites, e.g., the secretory activity and distribution of the peripheral vacuole system, but also homology with avian parasites, e.g., in nuclear and nucleolar structure and mitochondrial form. The latter homologies support earlier suggestions of the close phylogenetic relationship of P. falciparum with the avian parasites. Evidence is also presented showing the persistence of the cytoskeleton of the invasive sporozoite within the cytoplasm of the ensuing rapidly growing vegetative parasites.
哺乳动物疟原虫肝期形态培养能力，尤其是重要人类病原体恶性疟原虫的培养能力的最新进展，为研究该寄生虫在其天然宿主细胞——人类肝细胞中的超微结构组织提供了新机会。在这项电子显微镜和免疫荧光研究中，我们发现寄生虫和宿主细胞的形态均保存良好。可发现密度高达100个/平方厘米的红细胞外期形态，其生长速度与黑猩猩体内的生长速度相当。在繁殖期第5天和第7天的裂殖体中，寄生虫的超微结构组织与其他哺乳动物寄生虫有显著相似之处，例如外周泡系统的分泌活性和分布，但也与禽类寄生虫有同源性，例如在核和核仁结构以及线粒体形态方面。后者的同源性支持了早期关于恶性疟原虫与禽类寄生虫有密切系统发育关系的观点。还提供了证据表明侵入性子孢子的细胞骨架在随后快速生长的营养型寄生虫的细胞质中持续存在。
