Jiang Biao, Sun Yanxin, Li Wei, Liu Chun, Wen Caiyi, Li Anxing, Huang Yanhua, Su Youlu
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.
State Key Laboratory of Biocontrol/Guangdong Provincial Key Lab for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
Fish Shellfish Immunol. 2022 Apr;123:136-141. doi: 10.1016/j.fsi.2022.02.046. Epub 2022 Feb 23.
An experiment was performed to study the effects of dietary levels of black soldier fly larva meal (BSFLM) on the growth performance, immunity and disease resistance of juvenile grouper (Epinephelus coioides). Four isoproteic and isoenergetic diets were formulated with dietary BSFLM levels of 0 g/kg (T0), 25 g/kg (T2.5), 50 g/kg (T5) and 100 g/kg (T10). Each diet was randomly fed to triplicate groups, each containing 40 fish. The results of the 30-day study indicated that fish growth performance was not affected in the T2.5 and T5 groups compared with the T0 group. In the group with a dietary BSFLM level of 100 g/kg, the feed coefficient was significantly higher than that in the other three groups. The superoxide dismutase, catalase, glutathione peroxidase, lysozyme activity, and malondialdehyde content in the liver, and the interleukin-1 beta (IL-1β), gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α) and heat shock protein 70 (HSP70) expression in the gills, head kidney, liver and spleen remained consistent in all groups. In addition, no significant differences in the cumulative mortality or parasite abundance in groupers after Vibrio harveyi and Cryptocaryon irritans infection were observed. These results suggested that BSFLM supplemented diets did not inhibit disease resistance in groupers.
进行了一项实验，以研究黑水虻幼虫粉（BSFLM）日粮水平对幼鱼石斑鱼（Epinephelus coioides）生长性能、免疫力和抗病能力的影响。配制了四种等蛋白和等能量的日粮，日粮中BSFLM水平分别为0 g/kg（T0）、25 g/kg（T2.5）、50 g/kg（T5）和100 g/kg（T10）。每种日粮随机投喂给三个重复组，每组包含40条鱼。为期30天的研究结果表明，与T0组相比，T2.5组和T5组的鱼类生长性能没有受到影响。在日粮BSFLM水平为100 g/kg的组中，饲料系数显著高于其他三组。所有组中，肝脏中的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、溶菌酶活性和丙二醛含量，以及鳃、头肾、肝脏和脾脏中的白细胞介素-1β（IL-1β）、γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）和热休克蛋白70（HSP70）表达均保持一致。此外，在感染哈维氏弧菌和刺激隐核虫后，石斑鱼的累积死亡率或寄生虫丰度没有观察到显著差异。这些结果表明，添加BSFLM的日粮不会抑制石斑鱼的抗病能力。
