Nishio Tadashi, Toukairin Yoko, Hoshi Tomoaki, Arai Tomomi, Nogami Makoto
Department of Legal Medicine, Teikyo University School of Medicine, 2-11-1, Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
Department of Legal Medicine, Teikyo University School of Medicine, 2-11-1, Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
Leg Med (Tokyo). 2022 May;56:102047. doi: 10.1016/j.legalmed.2022.102047. Epub 2022 Feb 19.
Direct detection and accurate quantification of chlorine in autopsy samples are difficult because of the volatility and rapid metabolism of chlorine. Here, we developed and validated a method for quantitative analysis of 3-chloro-l-tyrosine (Cl-Tyr) and 3,5-dichloro-l-tyrosine (DiCl-Tyr) as stable markers of chlorine exposure. Chemical derivatization followed by liquid chromatography coupled with electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) enabled us to simultaneously analyze both Cl-Tyr and DiCl-Tyr in an autopsy sample from the victim of chlorine exposure. Cl-Tyr was detected in the heart blood (53.6 ng/mL), urine (9.5 ng/mL), and lung tissue (211.1 ng/g); however, DiCl-Tyr was detected only in the lung tissue (10.3 ng/g). In contrast, in autopsy samples obtained from cases without exposure to chlorine, DiCl-Tyr was not detected in any matrixes. Our result suggested that the simultaneous detection of Cl-Tyr and DiCl-Ty may provide a better appreciation of chlorine exposure. To our knowledge, this is the first time Cl-Tyr and DiCl-Tyr have been determined simultaneously in a real human autopsy sample from a victim of chlorine exposure.
由于氯的挥发性和快速代谢，对尸检样本中的氯进行直接检测和准确定量很困难。在此，我们开发并验证了一种用于定量分析3-氯-L-酪氨酸（Cl-Tyr）和3,5-二氯-L-酪氨酸（DiCl-Tyr）的方法，它们是氯暴露的稳定标志物。化学衍生化后，通过液相色谱与电喷雾电离串联质谱联用（LC/ESI-MS/MS），我们能够同时分析来自氯暴露受害者尸检样本中的Cl-Tyr和DiCl-Tyr。在心血（53.6 ng/mL）、尿液（9.5 ng/mL）和肺组织（211.1 ng/g）中检测到了Cl-Tyr；然而，仅在肺组织（10.3 ng/g）中检测到了DiCl-Tyr。相比之下，在未接触氯的病例的尸检样本中，在任何基质中均未检测到DiCl-Tyr。我们的结果表明，同时检测Cl-Tyr和DiCl-Ty可能有助于更好地了解氯暴露情况。据我们所知，这是首次在来自氯暴露受害者的真实人体尸检样本中同时测定Cl-Tyr和DiCl-Tyr。
