Institute for Glycomics, Griffith University, Gold Coast Campus, QLD, 4215, Australia.
Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD, 4111, Australia.
Biosens Bioelectron. 2022 Jun 1;205:114088. doi: 10.1016/j.bios.2022.114088. Epub 2022 Feb 19.
Recent advances in micro-electromechanical systems (MEMS) has allowed unprecedent perspectives for label-free detection (LFD) of biological and chemical analytes. Additionally, these LFD technologies offer the potential to design high resolution and high throughput sensing platforms, with the promise of further miniaturization. However, the immobilization of biomolecules onto inorganic surfaces without impacting their sensing abilities is crucial for designing these LFD technologies. Currently, covalent functionalization of self-assembled monolayers (SAMs) present promising pathways for improving assay sensitivity, reproducibility, surface stability and proximity of binding sites to the sensor surface. Herein, we investigate the use of chemical vapor deposition of 3-(glycidyloxypropyl)-trimethoxysilane (GOPTS) as a versatile SAM for the covalent functionalization of a SiO microcantilever array (MCA) for carbohydrate-lectin interactions with picogram sensitivity. Additionally, we demonstrate glycan immobilization to MCA is feasible using traditional piezoelectric microarray printer technology. Given the complexity of the glycome, the ability to spot samples in a high-throughput manner establishes our MCA as robust, label-free, and scalable means to analyze carbohydrate-protein interactions These findings demonstrate that GOPTS SAMs provide a suitable biofunctionalization route for MEMS and provides the proof of principle that can be extended to various LFD technologies toward a truly high-throughput and high-resolution platform.
微机电系统（MEMS）的最新进展为生物和化学分析物的无标记检测（LFD）提供了前所未有的前景。此外，这些 LFD 技术有潜力设计出高分辨率和高通量的感测平台，并有望进一步实现小型化。然而，将生物分子固定在无机表面上而不影响其感测能力对于设计这些 LFD 技术至关重要。目前，自组装单层（SAM）的共价功能化是提高分析灵敏度、重现性、表面稳定性和结合位点与传感器表面接近度的有前途的途径。在此，我们研究了使用 3-(缩水甘油氧基丙基）三甲氧基硅烷（GOPTS）的化学气相沉积作为一种通用的 SAM，用于共价功能化 SiO 微悬臂梁阵列（MCA）以实现对糖 - 凝集素相互作用的皮克灵敏度检测。此外，我们还证明了使用传统的压电微阵列打印机技术可以将聚糖固定在 MCA 上。鉴于聚糖的复杂性，以高通量方式点样的能力确立了我们的 MCA 作为分析碳水化合物 - 蛋白质相互作用的强大、无标记和可扩展的手段。这些发现表明 GOPTS SAM 为 MEMS 提供了一种合适的生物功能化途径，并提供了可以扩展到各种 LFD 技术的原理证明，以实现真正的高通量和高分辨率平台。
