Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong; Department of Mechanical and Energy Engineering, Southern University of Science and Technology, Shenzhen 518055, China.
Colloids Surf B Biointerfaces. 2022 May;213:112364. doi: 10.1016/j.colsurfb.2022.112364. Epub 2022 Jan 25.
Many biomaterials are made and studied to provide anticancer therapy, and many other biomaterials have been developed to assist body tissue regeneration. It has been a challenge to design and produce effective multifunctional, or bifunctional, biomaterials for clinical applications to prevent cancer recurrence and, at the same time, to promote new tissue formation after surgical removal of the tumor for millions of cancer patients. In this study, bifunctional UV and Sr double-crosslinked alginate (ALG)/allylated gelatin (GelAGE) hydrogels incorporated with polydopamine (PDA) particles were designed and made. Furthermore, doxorubicin hydrochloride (DOX), an anticancer drug, was incorporated in PDA particles. It was aimed for the new ALG/GelAGE-PDA@DOX hydrogels to exhibit anticancer synergy and hence provide combined chemotherapy and phototherapy (PTT) for bone tumor cell ablation. In vitro experiments using MG63 osteosarcoma cells showed that ALG/GelAGE-PDA@DOX hydrogels could effectively kill tumor cells through the synergy of controlled DOX release and hyperthermia ablation. It was also aimed for the new hydrogels to facilitate bone tissue regeneration at the original bone tumor site. The results of in vitro experiments demonstrated that owing to the release of Sr, the new hydrogels could promote the proliferation of rat bone mesenchymal stem cells (rBMSCs) and also the alkaline phosphatase (ALP) activity of cells, indicating their osteogenic promotion ability. The ALG/GelAGE-PDA@DOX hydrogels have therefore exhibited great potential for the treatment of bone tumor-related defects.
许多生物材料被制造和研究用于提供抗癌治疗，而许多其他生物材料也被开发出来以辅助身体组织再生。设计和生产用于临床应用的有效多功能或双功能生物材料一直是一个挑战，以防止癌症复发，同时促进数百万癌症患者在肿瘤切除手术后新组织的形成。在这项研究中，设计并制备了双功能 UV 和 Sr 双重交联海藻酸盐（ALG）/烯丙基化明胶（GelAGE）水凝胶，其中掺入了聚多巴胺（PDA）颗粒。此外，还将抗癌药物盐酸阿霉素（DOX）掺入 PDA 颗粒中。目的是使新的 ALG/GelAGE-PDA@DOX 水凝胶发挥协同抗癌作用，从而为骨肿瘤细胞消融提供联合化学疗法和光疗（PTT）。使用 MG63 骨肉瘤细胞进行的体外实验表明，ALG/GelAGE-PDA@DOX 水凝胶可以通过控制 DOX 释放和热疗消融的协同作用有效地杀死肿瘤细胞。其目的还在于使新水凝胶能够促进原始骨肿瘤部位的骨组织再生。体外实验结果表明，由于 Sr 的释放，新水凝胶能够促进大鼠骨髓间充质干细胞（rBMSCs）的增殖和细胞碱性磷酸酶（ALP）活性，表明其具有成骨促进能力。因此，ALG/GelAGE-PDA@DOX 水凝胶在治疗骨肿瘤相关缺损方面具有很大的潜力。
