Zeng Qiongyu, Han Yan, Li Haiyan, Chang Jiang
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
J Biomed Mater Res B Appl Biomater. 2014 Jan;102(1):42-51. doi: 10.1002/jbm.b.32978. Epub 2013 Jul 11.
Cell carrier is a useful biomedical tool to deliver particular kind of cells to a specific site for cell therapy or tissue regeneration. In the current study, 45S5 bioglass (BG) was introduced into alginate (ALG) to generate BG/ALG composite hydrogel beads as cell carriers. The ions releasing behavior, dimensional stability and in vitro bioactivity of the beads were investigated. Results showed that the BG/ALG beads revealed similar calcium ion releasing behavior as compared with ALG beads. In addition, silicon ion releasing was detected in BG/ALG beads. BG/ALG and ALG beads shared similar dimensional stability, and BG/ALG beads could induce apatite deposition on their surface after being soaked in stimulated body fluid. Then, the effects of ion extracts from hydrogel beads on cell behavior were investigated. Results confirmed that extracts of BG/ALG beads could simulate proliferation and osteogenic differentiation of mesenchymal stem cells as well as angiogenesis of endothelial cells. Furthermore, MC3T3-E1 cells were successfully encapsulated in hydrogel beads. BG/ALG beads enhanced the cell proliferation and stimulated osteogenic differentiation of the encapsulated MC3T3-E1 cells as compared with ALG beads. Therefore, BG/ALG composite hydrogel beads loaded with bone forming cells may be useful tools for bone regeneration and tissue engineering applications.
细胞载体是一种将特定类型的细胞输送到特定部位用于细胞治疗或组织再生的有用生物医学工具。在本研究中，将45S5生物玻璃（BG）引入海藻酸盐（ALG）中，以制备BG/ALG复合水凝胶珠作为细胞载体。研究了这些珠子的离子释放行为、尺寸稳定性和体外生物活性。结果表明，与ALG珠子相比，BG/ALG珠子表现出相似的钙离子释放行为。此外，在BG/ALG珠子中检测到了硅离子的释放。BG/ALG和ALG珠子具有相似的尺寸稳定性，并且BG/ALG珠子在浸泡于模拟体液后能够在其表面诱导磷灰石沉积。然后，研究了水凝胶珠离子提取物对细胞行为的影响。结果证实，BG/ALG珠子的提取物能够模拟间充质干细胞的增殖和成骨分化以及内皮细胞的血管生成。此外，MC3T3-E1细胞成功地封装在水凝胶珠中。与ALG珠子相比，BG/ALG珠子增强了封装的MC3T3-E1细胞的增殖并刺激了其成骨分化。因此，负载骨形成细胞的BG/ALG复合水凝胶珠可能是骨再生和组织工程应用的有用工具。
