从埃及健康奶牛及其饲养员中分离出的耐甲氧西林和甲氧西林敏感金黄色葡萄球菌克隆的分子特征分析。
Molecular characterisation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus clones isolated from healthy dairy animals and their caretakers in Egypt.
作者信息
El-Ashker Maged, Monecke Stefan, Gwida Mayada, Saad Thoraya, El-Gohary Adel, Mohamed Amro, Reißig Annett, Frankenfeld Katrin, Gary Dominik, Müller Elke, Ehricht Ralf
机构信息
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Leibniz-Institute of Photonic Technology (IPHT), Department for Optical Molecular Diagnostics and System Technology, Jena, Germany; InfectoGnostics Research Campus, Centre for Applied Research, Jena, Germany; Institute for Medical Microbiology and Virology, Dresden University Hospital, Dresden, Germany.
出版信息
Vet Microbiol. 2022 Apr;267:109374. doi: 10.1016/j.vetmic.2022.109374. Epub 2022 Feb 17.
The purpose of this study was to describe the clonal diversity of Staphylococcus aureus strains derived from healthy dairy cattle and buffaloes as well as their close contact caretakers from the Nile Delta region, Egypt during 2019 and 2020, and to determine their antimicrobial resistance genotypes and virulence determinants. The study included 360 samples (120 from each, dairy cattle, buffaloes and their contact caretakers) collected from eight smallholding dairy herds.The samples included udder skin swabs, composite milk samples and rectal swabs (40 samples each of bovines) and nasal swabs, hand swabs and stool specimens (40 samples each of caretakers). S. aureus were isolated by classical techniques and characterised using the DNA microarray technology. A total of 62 methicillin-resistant (MRSA) and 130 methicillin-sensitive (MSSA) S. aureus isolates were identified. MRSA carriage rate ranged between 2.5% - 15% (Mean: 10%) in dairy cattle, 5% - 15% (9.2%) in dairy buffaloes and 27.5% - 37.5% (30.8%) among the caretakers. Nine different clonal lineages of MRSA (including CC22, CC152, CC5, CC30, CC88, CC45, CC121, CC97, and CC15), and six clonal lineages of MSSA (CC97, CC50, CC188, CC361, CC15 and CC1278) were inferred. The study demonstrated, for the first time, a high clonal diversity of multi-drug resistant S. aureus clones (particularly CC152-MRSA-V, CC30-MRSA-IV, CC121-MRSA-V, CC15-MRSA-V, CC97-MRSA-PseudoSCCmec, CC361-MSSA and CC1278-MSSA) which colonise dairy cattle and buffaloes as well as their caretakers particularly in Damietta villages that located at the northern Mediterranean coast of Egypt. The findings highlight the potential dynamics of humans and animals' S. aureus strains which may represent a health threat for both populations. The complete absence of the lukM/lukF-P83 genes in the recovered isolates indicated that all recovered cattle isolates (except for CC97) were descendants of human lineages and that these replaced the original cow lineages. Hence, a recommendation was given to farm owners to review their hygiene regimen to help minimize the microbiological risks for both populations.
本研究的目的是描述2019年至2020年期间从埃及尼罗河三角洲地区健康奶牛和水牛及其密切接触的饲养员身上分离出的金黄色葡萄球菌菌株的克隆多样性，并确定其抗菌药物耐药基因型和毒力决定因素。该研究包括从八个小型奶牛场收集的360份样本（奶牛、水牛及其接触的饲养员各120份）。样本包括乳房皮肤拭子、混合乳样本和直肠拭子（牛各40份）以及鼻拭子、手拭子和粪便标本（饲养员各40份）。通过经典技术分离出金黄色葡萄球菌，并使用DNA微阵列技术进行鉴定。共鉴定出62株耐甲氧西林金黄色葡萄球菌（MRSA）和130株甲氧西林敏感金黄色葡萄球菌（MSSA）。奶牛中MRSA携带率在2.5%至15%之间（平均：10%），水牛中为5%至15%（9.2%），饲养员中为27.5%至37.5%（30.8%）。推断出9种不同的MRSA克隆谱系（包括CC22、CC152、CC5、CC30、CC88、CC45、CC121、CC97和CC15），以及6种MSSA克隆谱系（CC97、CC50、CC188、CC361、CC15和CC1278）。该研究首次证明，多药耐药金黄色葡萄球菌克隆（特别是CC152-MRSA-V、CC30-MRSA-IV、CC121-MRSA-V、CC15-MRSA-V、CC97-MRSA-PseudoSCCmec、CC361-MSSA和CC1278-MSSA）具有高度的克隆多样性，这些克隆定殖于奶牛和水牛及其饲养员身上，尤其是在埃及地中海沿岸北部的达米埃塔村庄。研究结果突出了人和动物金黄色葡萄球菌菌株的潜在动态，这可能对这两个群体都构成健康威胁。回收的分离株中完全不存在lukM/lukF-P83基因，这表明所有回收的牛分离株（CC97除外）都是人类谱系的后代，并且这些后代取代了原来的奶牛谱系。因此，建议农场主审查其卫生制度，以帮助将两个群体的微生物风险降至最低。
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