科威特医院中耐甲氧西林金黄色葡萄球菌（MSSA）分离株的基因组分析。

Genomic profiling of methicillin-sensitive (MSSA) isolates in Kuwait hospitals.

作者信息

Boswihi Samar S, Alfouzan Wadha A, Udo Edet E

机构信息

Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait.

Microbiology Unit, Department of Laboratories, Farwaniya Hospital, Farwaniya, Kuwait.

出版信息

Front Microbiol. 2024 Jul 17;15:1361217. doi: 10.3389/fmicb.2024.1361217. eCollection 2024.

DOI:10.3389/fmicb.2024.1361217

PMID:39086653

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11288847/

Abstract

BACKGROUND

is an important pathogen that causes mild to invasive infections in hospitals and the community. Although methicillin-susceptible (MSSA) isolates continue to cause different infections, there is no data on the genetic backgrounds of the MSSA colonizing or causing infections in Kuwait hospitals. This study aimed to investigate MSSA isolated from patients admitted to Kuwait hospitals for antibiotic resistance and genetic backgrounds to understand their clonal composition.

METHODS

Consecutive MSSA isolates were collected from single patients during two surveillance periods in 2016 and 2021 in 13 public hospitals. The isolates were characterized using antibiogram, staphylococcal protein A () typing, DNA microarray analysis, and multilocus sequence typing (MLST) using standard protocols.

RESULTS

A total of 446 MSSA was cultured from different clinical samples in 2016 ( = 240) and 2021 ( = 206). All isolates were susceptible to vancomycin [minimum inhibitory concentration (MIC) ≤ 2 mg/L], teicoplanin (MIC ≤2 mg/L), linezolid (MIC ≤4 mg/L), ceftaroline (MIC ≤2 mg/L), rifampicin, and mupirocin but were resistant to erythromycin (21.3%), clindamycin (14.0%), gentamicin (3.8%), kanamycin (10.5%), fusidic acid (27.0%), tetracycline (6.9%), trimethoprim (23.1%), and ciprofloxacin (35.2%). Molecular typing identified 155 types, dominated by t127 (15.0%), t084 (5.4%), t3841 (5.4%), t267 (2.4%), t442 (2.2%), t091 (2.2%), t021 (2.2%), and t003 (2.2%); 31 clonal complexes (CCs); and 56 sequence types (STs). The majority of the isolates ( = 265; 59.4%) belonged to CC1 (20.6%), CC15 (10.9%), CC22 (5.1%), CC30 (7.6%), CC361 (10.1%), and CC398 (4.7%).

DISCUSSION

The MSSA isolates belonged to diverse genetic backgrounds dominated by CC1, CC15, CC22, CC30, CC361, and CC398. The distribution of MSSA clones in 2016 and 2021 showed the stability of these clones over time. The study provides the first comprehensive data on the clonal distribution of MSSA in Kuwait hospitals.

摘要

背景

是一种重要的病原体，可在医院和社区引起从轻症到侵袭性感染。尽管甲氧西林敏感金黄色葡萄球菌（MSSA）分离株继续导致不同感染，但关于科威特医院中定殖或引起感染的MSSA的遗传背景尚无数据。本研究旨在调查从科威特医院入院患者中分离出的MSSA的抗生素耐药性和遗传背景，以了解其克隆组成。

方法

在2016年和2021年的两个监测期内，从13家公立医院的单个患者中连续收集MSSA分离株。使用抗菌谱、葡萄球菌蛋白A（）分型、DNA微阵列分析以及使用标准方案的多位点序列分型（MLST）对分离株进行鉴定。

结果

2016年（=240）和2021年（=206）共从不同临床样本中培养出446株MSSA。所有分离株对万古霉素[最低抑菌浓度（MIC）≤2mg/L]、替考拉宁（MIC≤2mg/L）、利奈唑胺（MIC≤4mg/L）、头孢洛林（MIC≤2mg/L）、利福平和平阳霉素敏感，但对红霉素（21.3%）、克林霉素（14.0%）、庆大霉素（3.8%）、卡那霉素（10.5%）、夫西地酸（27.0%）、四环素（6.9%）、甲氧苄啶（23.1%）和环丙沙星（35.2%）耐药。分子分型鉴定出155种类型，以t127（15.0%）、t084（5.4%）、t3841（5.4%）、t267（2.4%）、t442（2.2%）、t091（2.2%）、t021（2.2%）和t003（2.2%）为主；31个克隆复合体（CCs）；以及56种序列类型（STs）。大多数分离株（=265；59.4%）属于CC1（20.6%）、CC15（10.9%）、CC22（5.1%）、CC30（7.6%）、CC361（10.1%）和CC398（4.7%）。