Hepatic Surgery Department, The Third People's Hospital of Shenzhen, Shenzhen, Guangdong Province, China.
Department of Intensive Care Unit, The Third People's Hospital of Shenzhen, Shenzhen, Guangdong Province, China.
Bioengineered. 2022 Mar;13(3):6600-6614. doi: 10.1080/21655979.2022.2044260.
Circular RNAs (circRNAs) are non-coding RNAs with covalently closed structures that modulate the progression of hepatocellular carcinoma (HCC). Here, we explored whether circ_0008043 regulated the biological function of HCC cells. Quantitative real-time polymerase chain reaction (qPCR) was used to detect circ_0008043, microRNA (miR)-326, and RAB21 levels. Expression of E-cadherin, N-cadherin, and vimentin was assessed using qPCR. Cell proliferation, migration, and invasion were evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, and transwell assays. Xenograft tumors were used to evaluate cell growth . The interaction between miR-326 and circ_0008043 or RAB21 was assessed using dual-luciferase reporter analysis and RNA pull-down analysis. The data illustrated that circ_0008043 and RAB21 were highly expressed, while miR-326 was expressed at less levels in HCC tissues and cells. Interfering with circ_0008043 suppressed cellular proliferation, migration, invasion, and cell growth. Circ_0008043 was confirmed to be an miR-326 sponge that targets RAB21. Rescue experiments showed that inhibiting miR-326 abrogated the effect induced by knockdown of circ_0008043, and overexpressed RAB21 abolished the effect induced by miR-326 overexpression. In summary, silencing of circ_0008043 impeded HCC progression by regulating the miR-326/RAB21 axis. These data suggest that circ_0008043 may have clinical value in the treatment of HCC.
环状 RNA（circRNAs）是具有共价闭合结构的非编码 RNA，可调节肝细胞癌（HCC）的进展。在这里，我们探讨了 circ_0008043 是否调节 HCC 细胞的生物学功能。使用实时定量聚合酶链反应（qPCR）检测 circ_0008043、microRNA（miR）-326 和 RAB21 的水平。使用 qPCR 评估 E-钙粘蛋白、N-钙粘蛋白和波形蛋白的表达。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐、集落形成和 Transwell 测定评估细胞增殖、迁移和侵袭。使用异种移植肿瘤评估细胞生长。使用双荧光素酶报告分析和 RNA 下拉分析评估 miR-326 与 circ_0008043 或 RAB21 之间的相互作用。数据表明，circ_0008043 和 RAB21 在 HCC 组织和细胞中高表达，而 miR-326 表达水平较低。干扰 circ_0008043 抑制细胞增殖、迁移、侵袭和细胞生长。circ_0008043 被证实是 miR-326 的海绵，可靶向 RAB21。挽救实验表明，抑制 miR-326 可消除 circ_0008043 敲低引起的效应，而过表达 RAB21 可消除 miR-326 过表达引起的效应。总之，沉默 circ_0008043 通过调节 miR-326/RAB21 轴抑制 HCC 进展。这些数据表明，circ_0008043 在 HCC 的治疗中可能具有临床价值。
