Feltch S M, Stuehr J E
Biochemistry. 1979 May 15;18(10):1996-2000. doi: 10.1021/bi00577a023.
Equations have been developed for the relaxation times for a variety of mechanisms involving enzyme isomerization coupled to proton transfers. The concentration and pH dependences of the relaxation time have been calculated and graphed for a number of representative mechanisms. We find that for most of the mechanisms examined, the relaxation time is not only pH but also strongly concentration dependent. The concentration dependence results from finite perturbations of the hydrogen ion concentration. For the systems tested, the relaxation time shows a clear concentration dependence at enzyme concentrations below 200 microM.
已经针对涉及与质子转移偶联的酶异构化的多种机制，推导出了弛豫时间的方程。对于许多代表性机制，计算并绘制了弛豫时间对浓度和pH的依赖性。我们发现，对于所研究的大多数机制，弛豫时间不仅取决于pH，还强烈依赖于浓度。浓度依赖性源于氢离子浓度的有限扰动。对于所测试的系统，在酶浓度低于200微摩尔时，弛豫时间呈现出明显的浓度依赖性。
