Quarto Natalina, Menon Siddharth, Griffin Michelle, Huber Julika, Longaker Michael T
Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, United States.
Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, United States.
Front Physiol. 2022 Feb 10;13:823661. doi: 10.3389/fphys.2022.823661. eCollection 2022.
As a basic science, craniofacial research embraces multiple facets spanning from molecular regulation of craniofacial development, cell biology/signaling and ultimately translational craniofacial biology. Calvarial sutures coordinate development of the skull, and the premature fusion of one or more, leads to craniosynostosis. Animal models provide significant contributions toward craniofacial biology and clinical/surgical treatments of patients with craniofacial disorders. Studies employing mouse models are costly and time consuming for housing/breeding. Herein, we present the establishment of a calvarial suture explant 2-D culture method that has been proven to be a reliable system showing fidelity with the harvesting procedure to isolate high yields of skeletal stem/progenitor cells from small number of mice. Moreover, this method allows the opportunity to phenocopying models of craniosynostosis and tamoxifen-induction of suture explants to trace clonal expansion. This versatile method tackles needs of large number of mice to perform calvarial suture research.
作为一门基础科学，颅面研究涵盖多个方面，从颅面发育的分子调控、细胞生物学/信号传导，到最终的转化颅面生物学。颅骨缝协调颅骨的发育，一条或多条颅骨缝过早融合会导致颅缝早闭。动物模型对颅面生物学以及颅面疾病患者的临床/外科治疗做出了重大贡献。使用小鼠模型进行研究在饲养/繁殖方面成本高昂且耗时。在此，我们介绍一种颅骨缝外植体二维培养方法的建立，该方法已被证明是一个可靠的系统，在从少量小鼠中分离高产率的骨骼干/祖细胞的收获过程中显示出保真度。此外，这种方法提供了模拟颅缝早闭模型以及用他莫昔芬诱导缝线外植体以追踪克隆扩增的机会。这种通用方法满足了进行颅骨缝研究对大量小鼠的需求。
