Kang Yang, Bai Minghui, Deng Liling, Fan Linbo, Wang Xing
Department of Pediatrics, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Iran J Public Health. 2021 Oct;50(10):1973-1982. doi: 10.18502/ijph.v50i10.7497.
This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity.
Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB).
Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, <0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/Smad3 signaling pathway, thus affecting cell proliferation.
miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children.
本研究旨在探讨miRNA-21在儿童哮喘发病机制中的作用及其与病情严重程度的相关性。
纳入2018年6月至2019年12月齐齐哈尔医学院附属第三医院收治的54例支气管哮喘患儿。同时纳入49例同期进行体检的健康儿童。采用qRT-PCR分析外周血血清中miR-21的表达。通过Spearman相关性分析和ROC曲线探讨儿童哮喘表达与病情严重程度的关系。体外培养支气管上皮细胞系,分为空白对照组、阴性对照组、miR-21抑制组和激活组。采用CCK-8法检测不同组处理后细胞增殖的变化。通过蛋白质印迹法(WB)评估细胞中TGF-β1/Smad信号通路蛋白的表达。
与健康儿童相比,哮喘患儿外周血血清中miR-21表达较高(<0.001)。miR-21表达与病情严重程度呈正相关(r=0.853,<0.001)。体外细胞实验结果表明,miR-21可促进支气管上皮细胞增殖,并可能参与调节TGF-β1/Smad3信号通路的表达,从而影响细胞增殖。
miRNA-21通过激活TGFβ1/Smad信号通路调节支气管上皮细胞增殖。且其与儿童哮喘病情严重程度呈正相关。