Moriwaka F
Hokkaido Igaku Zasshi. 1986 Mar;61(2):208-19.
The hypothesis that amyotrophic lateral sclerosis (ALS) might be the disorder of "motor nerve growth factor" has been proposed, being based on the results of the research in tissue culture. The evidence of putative growth factor for spinal motor neurons has been searched, but not found yet. To investigate the growth factor for cultured rat fetal ventral spinal cord neurons two experiments were carried out. One experiment was to evaluate the rat fetal age-related dependency or requirement of putative growth factor in fetal calf serum and non-neuronal cells in cultured ret fetal ventral spinal cord neurons. Another experiment was the evaluation of the possibility as the growth factor of insulin, 5-alpha dihydrotestosterone, epidermal growth factor and fibroblast growth factor for cultured rat fetal ventral spinal cord neurons. The following results were obtained from these experiments; Insulin was found to be acting like a growth factor for rat fetal ventral spinal cord neurons, showing neurite promoting effect and choline acetyltransferase activity stimulating effect. 5-alpha dihydrotestosterone had neurite promoting effect on cultured rat fetal ventral spinal cord neurons without choline acetyltransferase activity stimulating effect. Epidermal growth factor and fibroblast growth factor which are regarded as insulin-related growth factor did not show apparent neurotrophic effect on cultured rat fetal ventral spinal cord neurons. There was a big difference in dependency or requirement of putative growth factor in fetal calf serum and non-neuronal cell between cultured rat fetal 13-15 day old ventral spinal cord neurons and cultured rat fetal 16-18 day old fetal ventral spinal cord neurons. It is necessary to consider about the rat fetal age on the evaluation of the effect of putative growth factor for cultured rat fetal ventral spinal cord neurons.
基于组织培养的研究结果，有人提出肌萎缩侧索硬化症（ALS）可能是一种“运动神经生长因子”紊乱的疾病。人们一直在寻找脊髓运动神经元假定生长因子的证据，但尚未找到。为了研究培养的大鼠胎儿腹侧脊髓神经元的生长因子，进行了两项实验。一项实验是评估培养的大鼠胎儿腹侧脊髓神经元中胎牛血清和非神经元细胞中假定生长因子与大鼠胎儿年龄的相关性或需求。另一项实验是评估胰岛素、5-α二氢睾酮、表皮生长因子和成纤维细胞生长因子作为培养的大鼠胎儿腹侧脊髓神经元生长因子的可能性。从这些实验中获得了以下结果：发现胰岛素对大鼠胎儿腹侧脊髓神经元起生长因子的作用，表现出促进神经突生长的作用和刺激胆碱乙酰转移酶活性的作用。5-α二氢睾酮对培养的大鼠胎儿腹侧脊髓神经元有促进神经突生长的作用，但没有刺激胆碱乙酰转移酶活性的作用。被认为是胰岛素相关生长因子的表皮生长因子和成纤维细胞生长因子对培养的大鼠胎儿腹侧脊髓神经元没有明显的神经营养作用。培养的大鼠胎儿13 - 15日龄腹侧脊髓神经元与培养的大鼠胎儿16 - 18日龄腹侧脊髓神经元在胎牛血清和非神经元细胞中假定生长因子的依赖性或需求上存在很大差异。在评估假定生长因子对培养的大鼠胎儿腹侧脊髓神经元的作用时，有必要考虑大鼠胎儿的年龄。
