Sun Xiao-Li, Jia Chun-Yan, Tian Shou-le, Xu Wen-Yan, Wang Jin-Ping, Ran Kun, Shen Guang-Ning
Shandong Institute of Pomology, Tai'an 271018, Shandong, China.
Taishan Forest Pest Control and Quarantine Station, Tai'an 271018, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2022 Jan;33(1):104-110. doi: 10.13287/j.1001-9332.202201.021.
Methylglyoxal (MG) is a novel signaling molecule with multiple functions in plants. To explore the effects of MG on Chinese chestnut () under drought stress, two-year-old 'Huangpeng' chestnut seedlings were treated with 15% polyethylene glycol (PEG) coupled with MG or its scavenger N-acetyl-L-cys-teine (NAC). We measured the activities of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), and glyoxalase enzymes, including glyoxalase Ⅰ (GlyⅠ) and glyoxalase Ⅱ(GlyⅡ). Contents of antioxidants such as endogenous MG, malondialdehyde (MDA), HO, and O as well as the osmotic adjustment substances including proline (Pro), soluble sugar (SS), glycine betaine (GB) were also detected. The results showed that 0.5 mmol·L MG significantly increased the activities of antioxidant enzymes (SOD, POD, CAT, APX, GR) and glyoxalase enzymes (GlyⅠ, GlyⅡ) in leaves of chestnut seedlings under drought stress, elevated the contents of osmotic adjustment substances (Pro, SS, GB) and antioxidant substances (ASA, GSH), and reduced the contents of MG, MDA, HO, O and dehydroascorbate (DHA). Drought stress induced damages such as membrane lipid peroxidation and osmotic stress was alleviated by MG, leading to an overall improved adaptability of chestnut to drought stress. Moreover, the addition of MG scavenger NAC could reverse the effects induced by MG, indicating that MG had positive impacts on drought resistance of chestnut plants. Our study provided a theoretical basis for further exploring the mechanism of MG in alleviating drought stress induced symptoms in chestnut.
甲基乙二醛（MG）是一种在植物中具有多种功能的新型信号分子。为了探究MG对干旱胁迫下板栗（）的影响，用15%聚乙二醇（PEG）联合MG或其清除剂N - 乙酰 - L - 半胱氨酸（NAC）处理两年生‘黄棚’板栗幼苗。我们测定了抗氧化酶的活性，包括超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR），以及乙二醛酶的活性，包括乙二醛酶Ⅰ（GlyⅠ）和乙二醛酶Ⅱ（GlyⅡ）。还检测了抗氧化剂的含量，如内源性MG、丙二醛（MDA）、HO和O，以及渗透调节物质，包括脯氨酸（Pro）、可溶性糖（SS）、甘氨酸甜菜碱（GB）。结果表明，0.5 mmol·L MG显著提高了干旱胁迫下板栗幼苗叶片中抗氧化酶（SOD、POD、CAT、APX、GR）和乙二醛酶（GlyⅠ、GlyⅡ）的活性，提高了渗透调节物质（Pro、SS、GB）和抗氧化物质（ASA、GSH）的含量，并降低了MG、MDA、HO、O和脱氢抗坏血酸（DHA）的含量。MG缓解了干旱胁迫诱导的膜脂过氧化和渗透胁迫等损伤，从而总体上提高了板栗对干旱胁迫的适应性。此外，添加MG清除剂NAC可逆转MG诱导的效应，表明MG对板栗植株的抗旱性有积极影响。我们的研究为进一步探究MG缓解板栗干旱胁迫诱导症状的机制提供了理论依据。
