Preclinical Surgical Research Laboratory, C. Wayne McIlwraith Translational Medicine Institute, Department of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.
Orthopaedic Bioengineering Research Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, USA.
Cartilage. 2022 Jan-Mar;13(1):19476035221081465. doi: 10.1177/19476035221081465.
Shoulder pain is commonly attributed to rotator cuff injury or osteoarthritis. Ovine translational models are used to investigate novel treatments aimed at remedying these conditions to prevent articular cartilage degeneration and subsequent joint degradation. However, topographical properties of articular cartilage in the ovine shoulder are undefined. This study investigates the biomechanical, morphological, and biochemical attributes of healthy ovine humeral head articular cartilage and characterizes topographical variations between surface locations.
Ten humeral heads were collected from healthy skeletally mature sheep and each was segregated into 4 quadrants using 16 regions of interest (ROIs) across the articular surface. Articular cartilage of each ROI was analyzed for creep indentation, thickness, and sulfated glycosaminoglycan (sGAG) and collagen quantity. Comparisons of each variable were made between quadrants and between ROIs within each quadrant.
Percent creep, thickness, and sGAG content, but not collagen content, were significantly different between humeral head quadrants. Subregion analysis of the ROIs within each surface quadrant revealed differences in all measured variables within at least one quadrant. Percent creep was correlated with sGAG (r = -0.32, = 0.0001). Collagen content was correlated with percent creep (r = 0.32, = 0.0009), sGAG (r = -0.19, = 0.049), and thickness (r = -0.19, = 0.04).
Topographical variations exist in mechanical, morphologic, and biochemical properties across the articular surface of the ovine humeral head. Recognizing this variability in ovine humeral head cartilage will provide researchers and clinicians with accurate information that could impact study outcomes.
肩部疼痛通常归因于肩袖损伤或骨关节炎。绵羊翻译模型用于研究旨在治疗这些疾病的新疗法，以防止关节软骨退化和随后的关节退化。然而，绵羊肩部关节软骨的形貌特征尚不清楚。本研究调查了健康绵羊肱骨头关节软骨的生物力学、形态和生化特性，并对关节表面不同位置的形貌变化进行了特征描述。
从健康成熟的绵羊中收集了 10 个肱骨头，每个肱骨头被分成 4 个象限，在关节表面使用 16 个感兴趣区域（ROI）。每个 ROI 的关节软骨进行蠕变压痕、厚度以及硫酸糖胺聚糖（sGAG）和胶原含量分析。在象限之间和每个象限内的 ROI 之间比较了每个变量。
肱骨头象限之间的蠕变百分比、厚度和 sGAG 含量差异显著，但胶原含量无差异。每个表面象限内 ROI 的亚区分析显示，在至少一个象限中，所有测量变量都存在差异。蠕变百分比与 sGAG 呈负相关（r = -0.32，p = 0.0001）。胶原含量与蠕变百分比（r = 0.32，p = 0.0009）、sGAG（r = -0.19，p = 0.049）和厚度（r = -0.19，p = 0.049）呈负相关。
在绵羊肱骨头关节表面存在机械、形态和生化特性的形貌变化。认识到绵羊肱骨头软骨的这种变异性将为研究人员和临床医生提供准确的信息，这可能会影响研究结果。
