College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
College of Science, Hebei Agricultural University, Baoding, Hebei 071000, China.
J Agric Food Chem. 2022 Mar 9;70(9):3023-3032. doi: 10.1021/acs.jafc.2c00337. Epub 2022 Feb 28.
In this study, a type of magnetic photoaffinity-labeled activity-based protein profiling probe for sulfonamide drugs was first synthesized for the purpose of capturing the natural dihydropteroate synthase of e by using simple incubation and magnetic separation. After characterization of its identity with LC-ESI-MS/MS, this enzyme was used as a recognition reagent to develop a direct competitive pseudo-ELISA for the determination of the residues of 40 sulfonamides in pork. Because of the use of streptavidin-horseradish peroxidase and biotinylated horseradish peroxidase as a signal-amplified system, the limits of detection for the 40 drugs were in the range of 0.001-0.016 ng/mL. Compared to the steps in a conventional assay formation, the operation steps were the same, but the sensitivities increased 32-88-fold. Furthermore, the assay performances were better than the previously reported immunoassays performances for sulfonamides. Therefore, this method could be used as a practical tool for multiscreening the trace levels of sulfonamides residues in food samples.
在这项研究中，我们首次合成了一种用于捕获 e 天然二氢喋呤合酶的磁性光亲和标记活性蛋白质分析探针，方法是简单孵育和磁分离。通过 LC-ESI-MS/MS 对其进行鉴定后，将该酶用作识别试剂，开发了一种直接竞争伪 ELISA 方法，用于测定猪肉中 40 种磺胺类药物的残留量。由于使用链霉亲和素-辣根过氧化物酶和生物素化辣根过氧化物酶作为信号放大系统，40 种药物的检测限在 0.001-0.016ng/mL 范围内。与传统测定方法的步骤相比，操作步骤相同，但灵敏度提高了 32-88 倍。此外，该测定方法的性能优于先前报道的磺胺类药物免疫测定方法的性能。因此，该方法可用作食品样品中痕量磺胺类药物残留的多筛查实用工具。
