一种基于金纳米粒子辅助小分子卟啉适体选择的 DNAzyme 开发方法。

A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme.

机构信息

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, China.

CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China.

出版信息

Methods Mol Biol. 2022;2439:3-13. doi: 10.1007/978-1-0716-2047-2_1.

DOI:10.1007/978-1-0716-2047-2_1

PMID:35226311

Abstract

DNAzymes can be obtained by in vitro selection, when specific analytes such as porphyrins are used as targets. In this process, the immobilization of targets serving as a major step is not suitable for the selection of DNAzymes because small molecules like porphyrins lack active groups for immobilization. Here, we develop a gold nanoparticle (AuNPs)-assisted aptamer selection for the small molecule porphyrin Zinc(II)-Protoporphyrin IX (ZnPPIX), which can realize the selection of DNAzyme without an immobilization step, and the detailed in vitro selection process in solution is described.

摘要

DNA 酶可以通过体外选择获得，当特定的分析物如卟啉类物质被用作靶标时。在这个过程中，作为主要步骤的靶标固定化不适合 DNA 酶的选择，因为像卟啉类物质这样的小分子缺乏用于固定化的活性基团。在这里，我们开发了一种金纳米粒子 (AuNPs) 辅助的小分子卟啉锌(II)-原卟啉 IX (ZnPPIX) 的适体选择，它可以实现无固定化步骤的 DNA 酶选择，并描述了溶液中详细的体外选择过程。