State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, 200237, China.
Analyst. 2009 Jul;134(7):1355-60. doi: 10.1039/b900900k. Epub 2009 Apr 2.
Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 5'-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules.
基于金纳米粒子（AuNPs）和工程化的 DNA 适体，我们设计了一种新颖的生物分析策略，用于检测作为小分子靶标的腺苷。在这个设计中，适体被设计为由两条通过其 5'-巯基修饰末端分别连接到 AuNPs 的无规卷曲状 ssDNA 片段组成。在存在特定靶标的情况下，它们可以重新组装成完整的适体三级结构并诱导纳米颗粒聚集。结果表明，金纳米粒子可以通过其基于特征表面等离子体共振的颜色变化有效地区分这两种不同的 DNA 结构。通过这种方法，可以在低微摩尔范围内选择性地检测腺苷，这意味着这里报道的策略可适用于检测其他几种小分子靶标。
