Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
Methods Mol Biol. 2022;2439:47-63. doi: 10.1007/978-1-0716-2047-2_4.
The efficiency of RNA-cleaving DNAzymes depends on a large extent on complex formation with their RNA targets. We describe available prediction tools that should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10-23 DNAzyme, but the procedure works as well for the 8-17 DNAzyme family.
RNA 切割型 DNA 酶的效率在很大程度上取决于与它们的 RNA 靶标形成复合物。我们描述了可用的预测工具，这些工具应该有助于设计高效的 DNA 酶，并展示了一些实验方法来验证这些预测。主要示例是 10-23 DNA 酶，但该程序也适用于 8-17 DNA 酶家族。
