Institute of Biophysics, Goethe University Frankfurt, Max-von-Laue-Straße 1, 60438, Frankfurt (Main), Germany.
Angew Chem Int Ed Engl. 2022 May 16;61(21):e202200648. doi: 10.1002/anie.202200648. Epub 2022 Apr 6.
Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role for energy dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET is required to elucidate its role in such processes. Ultrafast VIS-pump/IR-probe spectroscopy can detect pathways of VET in proteins. However, the requirement of having a VET donor and a VET sensor installed simultaneously limits the possible target proteins and sites; to increase their number we compare six IR labels regarding their utility as VET sensors. We compare these labels in terms of their FTIR, and VET signature in VET donor-sensor dipeptides in different solvents. Furthermore, we incorporated four of these labels in PDZ3 to assess their capabilities in more complex systems. Our results show that different IR labels can be used interchangeably, allowing for free choice of the right label depending on the system under investigation and the methods available.
振动能量转移 (VET) 作为蛋白质功能的关键机制正在出现，可能在能量耗散、别构调节和酶催化中发挥重要作用。为了阐明其在这些过程中的作用，需要深入了解 VET。超快可见泵浦/红外探针光谱学可用于检测蛋白质中的 VET 途径。然而，同时需要安装 VET 供体和 VET 传感器的要求限制了可能的目标蛋白质和位点；为了增加它们的数量，我们比较了六个 IR 标签，以评估它们作为 VET 传感器的实用性。我们根据它们在不同溶剂中的 FTIR 和 VET 供体-传感器二肽中的 VET 特征来比较这些标签。此外，我们将其中四个标签掺入 PDZ3 中，以评估它们在更复杂系统中的能力。我们的结果表明，不同的 IR 标签可以互换使用，根据所研究的系统和可用的方法，可以自由选择合适的标签。
