Plant Biοtechnοlοgy Department, Genetic Engineering and Biοtechnοlοgy Research Institute (GEBRI), University οf Sadat City, 79/22857 Sadat City, Egypt.
Department οf Pharmacοgnοsy, Faculty οf Pharmacy, Al-Azhar University, 11884 Nasr city, Cairο, Egypt.
Front Biosci (Landmark Ed). 2022 Jan 24;27(2):45. doi: 10.31083/j.fbl2702045.
(L.f.) Baker (Hyacinthaceae) is a perennial bulbous medicinal plant that is currently at risk of extinction. Squill (white sea onion) is an analogous cardiotonic to digitalis. The purpose of the current work was to assess the optimal growth conditions for cells to synthesize the cardiac glycoside proscillaridin A by involving illumination, carbon source, methyl jasmonate (MJ), and culture system.
When cells were cultured for 28 days at 21 ± 2 ∘C in the dark on Murashige and Skoog (MS) media containing 1 mg/L 2, 4-D and 0.5 mg/L Kin and 30 g/L sucrose, the cell proliferation, and proscillaridin A synthesis were effectively controlled. At low concentrations, MJ stimulated the synthesis of proscillaridin A (PsA). High-performance liquid chromatography (HPLC) analysis of suspension extracts demonstrated that the callus maintained in MS media enriched with 1 mg/L 2, 4-D and 0.5 mg/L Kin yielded a greater formation of Proscillaridin A (141.31 mg/g DW) than untreated plants.
The results indicate that cultures of may be an excellent source of proscillaridin A. Moreover, it is one of the most important cardiac glycoside, which has been found to exhibit anticancer activities. Suspension cultures of cells could be as highly productive as a callus culture.
（L.f.）贝克（风信子科）是一种多年生鳞茎药用植物，目前正面临灭绝的危险。海葱（白海葱）是一种类似于洋地黄的强心苷。本研究的目的是评估细胞合成强心苷普罗沙林 A 的最佳生长条件，涉及光照、碳源、茉莉酸甲酯（MJ）和培养系统。
当细胞在 21±2 ∘C 的黑暗条件下，在含有 1mg/L 2,4-D 和 0.5mg/L Kin 和 30g/L 蔗糖的 Murashige 和 Skoog (MS) 培养基中培养 28 天时，细胞增殖和普罗沙林 A 的合成得到了有效控制。低浓度的 MJ 刺激普罗沙林 A（PsA）的合成。悬浮提取物的高效液相色谱（HPLC）分析表明，在含有 1mg/L 2,4-D 和 0.5mg/L Kin 的 MS 培养基中维持的愈伤组织产生了更多的普罗沙林 A（141.31mg/gDW），比未处理的植物多。
结果表明，可能是普罗沙林 A 的一个极好来源。此外，它是最重要的强心苷之一，已被发现具有抗癌活性。细胞的悬浮培养可以像愈伤组织培养一样具有高生产力。
