AALZ-Aachen Dental Laser Center, RWTH Aachen University, 52074 Aachen, Germany.
Department of Orthodontics, Faculty of Medicine, Justus Liebig University Giessen, D-35392 Giessen, Germany.
Front Biosci (Landmark Ed). 2022 Feb 14;27(2):62. doi: 10.31083/j.fbl2702062.
Dental avulsion due to trauma, especially in young patients, is a worldwide problem, requiring tooth replacement. Delayed replantation could cause tooth loss when the cementum is severely damaged. A small number of studies has reported that photobiomodulation (PBM) therapy using Er: YAG laser irradiation activates cellular signaling responses in different cell types, resulting in a variety of favorable biological effects. The aim of this study was to evaluate the potential biostimulatory effect of low-level Er: YAG laser irradiation on the biological responses of cultured mouse cementoblasts (OCCM-30), including the mitogen-activated protein kinases (MAPKs).
OCCM-30 cells were exposed to 2940 nm Er: YAG laser irradiation for 15 s at 0.34 W (pulse duration of 100 or 1000 μs, 17 mJ/pulse) at energy densities of 1 or 2 J/cm. Irradiated and non-irradiated OCCM-30 cells were tested for migration (Scratch assay), proliferation (MTS assay) and functional differentiation (Alizarin Red S assay). () and () gene expression, and activation of MAPKs, were assessed by RT-PCR and Western blotting, respectively.
Low-level Er: YAG laser irradiation at 2 J/cm and pulse duration of 1000 μs resulted in the highest migration rate and proliferation. Moreover, the pulse duration irradiation of 100 μs increased expression. expression was increased after 1000 μs pulse duration laser stimulation. Low-level Er: YAG laser irradiation increased the mineralization of OCCM-30 cells after 7 days and activated ERK1/2, P38 and JNK signaling.
Low-level Er: YAG laser irradiation induces OCCM-30 cell migration, proliferation and differentiation, and activates the MAPK signaling pathway.
外伤性牙齿脱落，尤其是在年轻患者中，是一个全球性的问题，需要进行牙齿修复。如果牙骨质受到严重损伤，延迟再植可能会导致牙齿脱落。一些研究报道，使用铒：YAG 激光辐照的光生物调节（PBM）疗法可激活不同细胞类型中的细胞信号转导反应，从而产生多种有利的生物学效应。本研究旨在评估低水平铒：YAG 激光辐照对培养的小鼠成牙骨质细胞（OCCM-30）的生物学反应的潜在生物刺激作用，包括丝裂原激活的蛋白激酶（MAPKs）。
OCCM-30 细胞分别用脉冲持续时间为 100 或 1000 μs、能量密度为 1 或 2 J/cm2 的 2940nm 铒：YAG 激光辐照 15s（功率为 0.34W，脉冲 17mJ/pulse）。通过划痕试验、MTS 试验和茜素红 S 试验检测辐照和未辐照的 OCCM-30 细胞的迁移、增殖和功能分化。通过 RT-PCR 和 Western blot 分别检测 () 和 () 基因表达和 MAPKs 的激活。
2 J/cm2 能量密度和 1000 μs 脉冲持续时间的低水平铒：YAG 激光辐照可获得最高的迁移率和增殖率。此外，100μs 脉冲持续时间的辐照增加了 () 表达。1000μs 脉冲持续时间激光刺激后 () 表达增加。低水平铒：YAG 激光辐照可增加 OCCM-30 细胞 7 天后的矿化，并激活 ERK1/2、P38 和 JNK 信号通路。
低水平铒：YAG 激光辐照可诱导 OCCM-30 细胞迁移、增殖和分化，并激活 MAPK 信号通路。
