Navarro E, Bach M, Durán N, Puigdomènech P, Palau J
J Immunol Methods. 1986 Jul 11;91(1):75-81. doi: 10.1016/0022-1759(86)90104-3.
A multiple sample immunoblotting system (MSIS) is described. The MSIS permits the detection and classification of antinuclear antibodies (ANA) according to the pattern of antigenic polypeptides recognized in four different extracts: calf thymus whole tissue, nuclear extract, and two ammonium sulphate fractions of the nuclear extract. The procedure permits the classification of anti-RNP, anti-SS/B and anti-Sm, and the detection of new ANAs in sera from SLE patients. The reaction patterns presented are specific and unambiguous, and make the use of validated control sera unnecessary.
本文描述了一种多样本免疫印迹系统（MSIS）。该MSIS可根据在四种不同提取物（小牛胸腺全组织、核提取物以及核提取物的两个硫酸铵级分）中识别的抗原多肽模式，对抗核抗体（ANA）进行检测和分类。该方法可对抗RNP、抗SS/B和抗Sm进行分类，并检测系统性红斑狼疮（SLE）患者血清中的新型ANA。所呈现的反应模式具有特异性且明确，无需使用经过验证的对照血清。
