Kim Youn Ju, Kim Hye Jin, Lee Sang Gyu, Kim Do Hyun, In Jang Su, Go Hye Sun, Lee Won Jun, Seong Je Kyung
Laboratory of Developmental Biology and Genomics, BK21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea; The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea; Korea Mouse Phenotyping Center (KMPC), Seoul National University, 08826 Seoul, Republic of Korea.
The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea; Korea Mouse Phenotyping Center (KMPC), Seoul National University, 08826 Seoul, Republic of Korea.
Metabolism. 2022 May;130:155178. doi: 10.1016/j.metabol.2022.155178. Epub 2022 Feb 25.
The relationship between exercise training and health benefits is under thorough investigation. However, the effects of exercise training on the maintenance of metabolic health are unclear.
Our experimental design involved initial exercise training followed by a high-fat diet (HFD) challenge. Eight-week-old male was trained under voluntary wheel running aerobic exercise for eight weeks to determine the systemic metabolic changes induced by exercise training and whether such changes persisted even after discontinuing exercise. The mice were given either a normal chow diet (NCD) or HFD ad libitum for one week after discontinuation of exercise (CON-NCD, n = 29; EX-NCD, n = 29; CON-HFD, n = 30; EX-HFD, n = 31).
Our study revealed that metabolic stress following the transition to an HFD in mice that discontinued training failed to reverse the aerobic exercise training-induced improvement in metabolism. We report that the mice subjected to exercise training could better counteract weight gain, adipose tissue hypertrophy, insulin resistance, fatty liver, and mitochondrial dysfunction in response to an HFD compared with untrained mice. This observation could be attributed to the fact that exercise enhances the browning of white fat, whole-body oxygen uptake, and heat generation. Furthermore, we suggest that the effects of exercise persist due to PPARα-FGF21-FGFR1 mechanisms, although additional pathways cannot be excluded and require further research. Although our study suggests the preventive potential of exercise, appropriate human trials are needed to demonstrate the efficacy in subjects who cannot perform sustained exercise; this may provide an important basis regarding human health.
运动训练与健康益处之间的关系正在深入研究中。然而，运动训练对维持代谢健康的影响尚不清楚。
我们的实验设计包括先进行运动训练，然后进行高脂饮食（HFD）挑战。对8周龄雄性小鼠进行为期8周的自愿轮转有氧运动训练，以确定运动训练引起的全身代谢变化，以及即使停止运动后这些变化是否仍然持续。运动停止后，小鼠自由摄取普通饲料（NCD）或HFD一周（CON-NCD，n = 29；EX-NCD，n = 29；CON-HFD，n = 30；EX-HFD，n = 31）。
我们的研究表明，停止训练的小鼠在转换为HFD后出现的代谢应激未能逆转有氧运动训练引起的代谢改善。我们报告，与未训练的小鼠相比，接受运动训练的小鼠在面对HFD时能够更好地抵抗体重增加、脂肪组织肥大、胰岛素抵抗、脂肪肝和线粒体功能障碍。这一观察结果可能归因于运动增强了白色脂肪的褐色化、全身氧气摄取和热量产生。此外，我们认为运动的影响通过PPARα-FGF21-FGFR1机制持续存在，尽管不能排除其他途径，需要进一步研究。虽然我们的研究表明了运动的预防潜力，但需要适当的人体试验来证明对无法进行持续运动的受试者的疗效；这可能为人类健康提供重要依据。
