脉冲电场对狭鳕鱼卵提取总脂质产率和脂质组学特征的影响。
The effect of pulsed electric fields on the extracted total lipid yield and the lipidomic profile of hoki roe.
机构信息
Department of Food Science, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand; Department of Fishing and Post-harvest Technology, Faculty of Fisheries, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225, Bangladesh.
Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand.
出版信息
Food Chem. 2022 Aug 1;384:132476. doi: 10.1016/j.foodchem.2022.132476. Epub 2022 Feb 17.
The effect of pulsed electric fields (PEF) at different field strengths (0.62, 1.25, 1.875 kV/cm) and frequencies (25, 50, 100 Hz) on total lipid extraction from hoki roe was investigated, along with the lipidomic profile (total lipid, phospholipid, fatty acid, phospholipid composition, and positional distribution of EPA and DHA). High PEF input (112 kJ/kg, 1.875 kV/cm and 100 Hz) yielded the highest total lipid (16.2% wet weight (WW)), and phospholipid (46 μmol/g WW) contents, without affecting n-3 fatty acid content (32%), and generated the highest LDPG, LPE, LPS and LPC contents (1.1, 0.41, 6.13 and 2.15 μmol/g WW, respectively). However, this PEF treatment resulted in sn-2 phospholipid EPA and DHA to be relocated to the sn-1,3 positions. Despite the good yield of n-3 fatty acids and PL, high PEF intensity treatment was found to result in negative structural changes in hoki roe lipids.
研究了不同电场强度（0.62、1.25、1.875 kV/cm）和频率（25、50、100 Hz）的脉冲电场（PEF）对鳕鱼鱼卵总脂质提取的影响，以及脂质组学谱（总脂质、磷脂、脂肪酸、磷脂组成和 EPA 和 DHA 的位置分布）。高 PEF 输入（112 kJ/kg、1.875 kV/cm 和 100 Hz）产生了最高的总脂质（16.2%湿重（WW））和磷脂（46 μmol/g WW）含量，而不影响 n-3 脂肪酸含量（32%），并产生了最高的 LDPG、LPE、LPS 和 LPC 含量（分别为 1.1、0.41、6.13 和 2.15 μmol/g WW）。然而，这种 PEF 处理导致 sn-2 磷脂中的 EPA 和 DHA 重新定位到 sn-1,3 位置。尽管 n-3 脂肪酸和 PL 的产量很高，但发现高强度 PEF 处理会导致鳕鱼鱼卵脂质的结构发生负面变化。
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