Lehrer N, Segal E, Cihlar R L, Calderone R A
J Med Vet Mycol. 1986 Apr;24(2):127-31. doi: 10.1080/02681218680000191.
A spontaneous, cerulenin-resistant mutant of Candida albicans (strain 4918-10) was found to adhere less readily to human vaginal mucosal cells in vitro than a wild type C. albicans (strain 4918). In a murine model of vaginal infection, strain 4918-10 was found to be less virulent than wild type C. albicans, i.e., the infection rate caused by 4918-10 was only 31% of that observed with wild type, 4918. A chitin-soluble extract (CSE) prepared from 4918 blocked attachment of yeast cells to human vaginal epithelial cells, while CSE from 4918-10 did not significantly reduce the attachment of yeasts to vaginal cells. Both 4918 and 4918-10 produced hyphae in vitro and in vivo, were negative for proteinase production and grew equally well at 28 degrees C and 37 degrees C. The data suggest that adherence to vaginal mucosa may be an important determinant in the pathogenesis of vaginal infection caused by C. albicans.
发现白色念珠菌的一个自发、对浅蓝菌素耐药的突变体（菌株4918 - 10）在体外比野生型白色念珠菌（菌株4918）更不容易黏附于人阴道黏膜细胞。在阴道感染的小鼠模型中，发现菌株4918 - 10的毒力低于野生型白色念珠菌，即由4918 - 10引起的感染率仅为野生型4918的31%。从4918制备的几丁质可溶性提取物（CSE）可阻止酵母细胞黏附于人阴道上皮细胞，而来自4918 - 10的CSE并未显著降低酵母对阴道细胞的黏附。4918和4918 - 10在体外和体内均产生菌丝，蛋白酶产生呈阴性，且在28℃和37℃下生长同样良好。数据表明，黏附于阴道黏膜可能是白色念珠菌引起阴道感染发病机制中的一个重要决定因素。
