Nanjareddy Kalpana, Zepeda-Jazo Isaac, Arthikala Manoj-Kumar
Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León Universidad Nacional Autónoma de México (UNAM), León, C.P. 37689 Guanajuato Mexico.
Departamento de Genómica Alimentaria Universidad de La Ciénega del Estado de Michoacán de Ocampo, Sahuayo, C.P. 59103 Michoacán de Ocampo Mexico.
Appl Plant Sci. 2022 Feb 8;10(1):e11454. doi: 10.1002/aps3.11454. eCollection 2022 Jan-Feb.
-induced hairy root systems are one of the most preferred and versatile systems for the functional characterization of genes. The use of hairy root systems is a rapid and convenient alternative for studying root biology, biotic and abiotic stresses, and root symbiosis in in vitro recalcitrant legume species such as .
We present a rapid, simplified method for the generation of composite plants with transgenic hairy roots. We demonstrate a technique of hairy root induction mediated by from young shoots. The efficacy of the system for producing transgenic roots is demonstrated using an enhanced green fluorescent protein (eGFP) expression vector. Furthermore, the application of the system for studying root branching is shown using the auxin-responsive marker promoter fused to β-glucuronidase (GUS). Finally, the success of the hairy root system for root symbiotic studies is illustrated by inoculating hairy roots with arbuscular mycorrhizal fungi.
In this study, we have developed a rapid, efficient, and cost-effective composite plant protocol for that is particularly effective for root-related studies and for the validation of candidate genes in during mycorrhizal symbiosis.
发根农杆菌诱导的毛状根系是用于基因功能表征的最优选且通用的系统之一。对于研究诸如体外难转化豆科植物的根系生物学、生物和非生物胁迫以及根共生而言，使用毛状根系是一种快速且便捷的替代方法。
我们提出了一种用于生成具有转基因毛状根的复合植物的快速、简化方法。我们展示了一种由幼嫩茎段的发根农杆菌介导的毛状根诱导技术。使用增强型绿色荧光蛋白（eGFP）表达载体证明了该系统产生转基因根的功效。此外，使用与β-葡萄糖醛酸酶（GUS）融合的生长素响应标记启动子展示了该系统在研究根分支方面的应用。最后，通过用丛枝菌根真菌接种毛状根说明了毛状根系用于根共生研究的成功。
在本研究中，我们为某植物开发了一种快速、高效且经济高效的复合植物方案，该方案对于根相关研究以及在菌根共生期间验证某植物中的候选基因特别有效。
