硅酸钙基水门汀对间充质干细胞牙本质/成骨分化潜能的影响
Effects of calcium silicate-based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells.
作者信息
Sismanoglu Soner, Ercal Pinar
机构信息
Department of Restorative Dentistry, Faculty of Dentistry, Altınbaş University, Istanbul, Turkey.
Centre for Oral Immunobiology and Regenerative Medicine and Centre for Oral Clinical Research, Institute of Dentistry, Queen Mary University London (QMUL), London, UK.
出版信息
Aust Endod J. 2023 Apr;49(1):66-74. doi: 10.1111/aej.12615. Epub 2022 Mar 1.
The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells (TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.
本研究的目的是评估生物陶瓷（Biodentine）、新型氢氧化钙材料（NeoMTA Plus）和牙髓盖髓剂（TheraCal LC）对牙胚来源干细胞（TGSCs）的生物学效应和成牙/成骨分化潜能。将TGSCs暴露于材料提取物中。通过MTS细胞增殖试验检测生物相容性。通过碱性磷酸酶（ALP）活性和mRNA基因表达（RUNX2、DSPP和DMP-1）评估成牙/成骨分化。还对材料进行了扫描电子显微镜/能量色散X射线（SEM/EDX）分析和pH分析。使用单因素方差分析和Tukey检验对数据进行评估。与所有测试材料孵育7天后，TGSCs仍保持活力。与TheraCal LC相比，生物陶瓷和新型氢氧化钙材料显示出较高的ALP活性以及RUNX2、DSPP和DMP-1表达增加。所有材料均可在不同程度上诱导间充质干细胞的成牙/成骨分化。生物陶瓷和新型氢氧化钙材料的生物相容性和成牙/成骨分化潜能相似，且优于TheraCal LC。
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