Santiago Marcos Coelho, Henrique de Oliveira Salles Gustavo, Gomes de Lima Gustavo, Alves de Oliveira Laudimar, Salles Loise Pedrosa
University of Brasilia, Faculty of Health Sciences, Post-Graduation Program in Dentistry, Brasília, DF, Brazil.
University of Planalto Central, Medical School, Brasília, DF, Brazil.
J Oral Biol Craniofac Res. 2025 Jan-Feb;15(1):191-198. doi: 10.1016/j.jobcr.2025.01.008. Epub 2025 Jan 14.
The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic sealers (MTA-F).
All materials were prepared according to the manufacturer's instructions. Before exposing the cells, we measured the release of calcium ions (Ca) from the dental materials to the culture media once Ca can trigger signaling pathways. After that, hDPCs and Saos-2 were exposed to the sealers for MTT assay to assess the cell viability and wound healing to evaluate the cell proliferation. To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. The results were significant when p < 0.05.
The sealers released diverse concentrations of calcium at different times. The hDPCs viability and proliferation were low in the AHP-B group at 24h of exposure (NEOMTA-P ∼ BIO-C ∼ CT > AHP-B > MTA-F), distinct from the osteoblastic cells (NEOMTA-P ∼ AHP-B ∼ CT > BIO-C > MTA-F) and (proliferation: AHP-B > NEOMTA-P ∼ CT > BIO-C > MTA-F). The ALP activity, an early marker of osteogenesis, was higher in hDPCs exposed to NEOMTA-P, while the osteoblastic cells showed higher ALP when exposed to AHP-B.
AHP-B, NEOMTA-P, and BIO-C stimulated osteogenesis in hDPCs and Saos-2 cells, with marked differences between groups. AHP-B showed an improved early stimulation of osteoblastic cells, while hDPCs were more responsive to NEOMTA-P.
本研究旨在比较人牙髓细胞(hDPCs)和成骨细胞系(Saos-2)在暴露于AH Plus®生物陶瓷(AHP-B)、Bio-C®封闭剂(BIO-C)、NeoMTA Plus®(NEOMTA-P)和MTA-FILLAPEX®根管封闭剂(MTA-F)后的活力、增殖和矿化潜力。
所有材料均按照制造商的说明制备。在将细胞暴露之前,由于钙离子(Ca)可触发信号通路,我们测量了牙科材料向培养基中钙离子的释放量。之后,将hDPCs和Saos-2暴露于封闭剂中进行MTT试验以评估细胞活力,并进行伤口愈合试验以评估细胞增殖。为了研究矿化潜力,我们通过茜素红染色评估碱性磷酸酶活性和钙沉积。使用双向方差分析进行钙离子释放和伤口愈合试验的统计分析,使用单向方差分析进行其他试验的统计分析,并采用事后检验Bonferroni校正。当p < 0.05时,结果具有显著性。
封闭剂在不同时间释放出不同浓度的钙。在暴露24小时时,AHP-B组中hDPCs的活力和增殖较低(NEOMTA-P ∼ BIO-C ∼ CT > AHP-B > MTA-F),这与成骨细胞不同(NEOMTA-P ∼ AHP-B ∼ CT > BIO-C > MTA-F),(增殖情况:AHP-B > NEOMTA-P ∼ CT > BIO-C > MTA-F)。碱性磷酸酶活性是成骨的早期标志物,在暴露于NEOMTA-P的hDPCs中较高,而成骨细胞在暴露于AHP-B时碱性磷酸酶活性较高。
AHP-B、NEOMTA-P和BIO-C刺激了hDPCs和Saos-2细胞的成骨作用,各实验组之间存在显著差异。AHP-B对成骨细胞的早期刺激作用有所改善,而hDPCs对NEOMTA-P的反应更强。