Barkovskiĭ A L, Shub G M
Mikrobiologiia. 1986 Mar-Apr;55(2):237-40.
A bacterial strain was isolated from soil and identified as Acinetobacter calcoaceticus var. lwoffii. The strain can utilize a wide spectrum of aromatic compounds. It carries a transmissive plasmid pBSW13 which determines resorcin utilization via the ortho pathway including the following steps: resorcin-hydroxyhydroquinone-maleylacetate-beta-ketoadipi c acid. The plasmid has been transferred by conjugation into the recipient strains of A. calcoaceticus 5734 CCM rifr, Escherichia coli J-53 met-pro-rifr and Klebsiella sp. Plasmid DNA with a molecular mass close to that of phage gamma was detected by electrophoresis in the donor and recombinant strains. The degradation of other substrates is not a phenotypic expression of the genes of this plasmid.
从土壤中分离出一株细菌，鉴定为醋酸钙不动杆菌鲁氏变种。该菌株能够利用多种芳香族化合物。它携带一种可转移质粒pBSW13，该质粒通过邻位途径决定间苯二酚的利用，包括以下步骤：间苯二酚-羟基对苯二酚-马来酰乙酸-β-酮己二酸。该质粒已通过接合转移到醋酸钙不动杆菌5734 CCM rifr、大肠杆菌J-53 met-pro-rifr和克雷伯氏菌属的受体菌株中。通过电泳在供体菌株和重组菌株中检测到分子量与噬菌体γ接近的质粒DNA。其他底物的降解不是该质粒基因的表型表达。
