School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Anal Bioanal Chem. 2022 Apr;414(9):2935-2951. doi: 10.1007/s00216-022-03921-y. Epub 2022 Mar 1.
β-Glucuronidase (β-GLU), a kind of hydrolase, is widely distributed in mammalian tissues, body fluids, and microbiota. Abnormal changes of β-GLU activity are often correlated with the occurrence of diseases and deterioration of water quality. Therefore, detection of β-GLU activity is of great significance in biomedicine and environmental health such as cancer diagnosis and water monitoring. However, the conventional β-GLU activity assay suffers from the limitations of low sensitivity, poor accuracy, and complex procedure. With the development of analytical chemistry, many advances have been made in the detection of β-GLU activity in recent years. The sensors for β-GLU activity detection which have the advantages of rapid and reliable detection have been attracting increased attentions. In this paper, the principles, performances, and limitations of these β-GLU sensors, including colorimetric sensing, fluorescent sensing, electrochemical sensing for the determination of β-GLU activity, have been summarized and discussed. Moreover, the challenges and research trends of β-GLU activity assay are proposed.
β-葡萄糖醛酸酶（β-GLU）是一种水解酶，广泛分布于哺乳动物组织、体液和微生物群中。β-GLU 活性的异常变化通常与疾病的发生和水质恶化有关。因此，检测 β-GLU 活性在癌症诊断和水质监测等生物医学和环境健康领域具有重要意义。然而，传统的β-GLU 活性测定方法存在灵敏度低、准确性差和操作复杂等局限性。随着分析化学的发展，近年来在β-GLU 活性检测方面取得了许多进展。具有快速可靠检测优点的β-GLU 活性传感器引起了越来越多的关注。本文总结和讨论了用于测定β-GLU 活性的比色传感、荧光传感和电化学传感等β-GLU 传感器的原理、性能和局限性。此外，还提出了β-GLU 活性测定的挑战和研究趋势。
