Pak J Biol Sci. 2022 Jan;25(2):112-122. doi: 10.3923/pjbs.2022.112.122.
<b>Background and Objective:</b> Cholestasis is a liver disease that occurs when bile flow is restricted or blocked. Estrogen-induced cholestasis is marked by a reduction in bile flow and the accumulation of bile acids in the liver as well as liver damage. The aim was to evaluate the hepatoprotective effect on EE-induced cholestasis in rats of Cranberry Water Extract (CWE). <b>Materials and Methods:</b> Adult albino rats weighing approximately 150±10 g were divided into six groups of six animals each. As control groups, three groups (I, II and IV) and three experimental groups were used (III, V, VI). <b>Results:</b> Oral administration for 15 days of CWE (150 mg kg<sup>1</sup> b.wt.) in EE-treated rats (100 μg kg<sup>1</sup> 5 days b.wt.) improved serum cholesterol, bile acid and TBIL as well as hepatic SOD and GPx significantly. Also, CWE inhibited ALP, ALT, γ-GT activity as well as levels of TNF-α, NO, MMP-2 and MMP-9 and MDA in comparison with the EE treatment rats. On the other hand, the liver TLR4, NF-κB and p38MAPK gene expression was down regulated group of rats administrated with cranberry extract when compared with the EE-treated rats. CWE's prophylactic action II is more pronounced than prophylactic one. The hepatoprotective effects of cranberry in restoring normal liver functional ability were also supported by histopathological examination of liver tissues. <b>Conclusion:</b> The results show clearly that cranberry extract has a strong prophylactic effect in EE-induced cholestasis by normalizing the levels of TLR4, NF-κB and p38MAPK gene expression.
胆汁淤积是一种肝脏疾病，发生在胆汁流动受到限制或阻塞时。雌激素诱导的胆汁淤积症的特点是胆汁流量减少，肝脏中胆汁酸积聚以及肝损伤。本研究旨在评估蔓越莓水提取物（CWE）对 EE 诱导的大鼠胆汁淤积的肝保护作用。材料与方法：成年白化大鼠，体重约 150±10 g，分为六组，每组六只动物。作为对照组，使用三组（I、II 和 IV）和三组实验组（III、V、VI）。结果：EE 处理大鼠（100μgkg15 天 b.wt.）口服给予 CWE（150mgkg1b.wt.）15 天可显著改善血清胆固醇、胆汁酸和 TBIL 以及肝 SOD 和 GPx。此外，CWE 抑制 ALP、ALT、γ-GT 活性以及 TNF-α、NO、MMP-2 和 MMP-9 和 MDA 的水平，与 EE 处理大鼠相比。另一方面，与 EE 处理大鼠相比，给予蔓越莓提取物的大鼠 TLR4、NF-κB 和 p38MAPK 基因表达下调。CWE 的预防作用 II 比预防作用更明显。肝组织学检查也支持蔓越莓在恢复正常肝功能方面的肝保护作用。结论：研究结果表明，蔓越莓提取物通过使 TLR4、NF-κB 和 p38MAPK 基因表达正常化，对 EE 诱导的胆汁淤积具有很强的预防作用。
