Xiong Xiang, Liao Hao, Xing Yanfang, Han Xukun, Wang Wanle, Wan Wenjie, Huang Qiaoyun, Chen Wenli
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural Universitygrid.35155.37, Wuhan, China.
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural Universitygrid.35155.37, Wuhan, China.
Microbiol Spectr. 2022 Apr 27;10(2):e0252421. doi: 10.1128/spectrum.02524-21. Epub 2022 Mar 2.
Bacterial interactions play significant roles in ecological functions in responding to anthropogenic interference and soil structure changes. However, it remains largely unknown how fertilizer regimes and soil particle sizes drive bacterial interactions. To evaluate bacterial interaction patterns in soil aggregates under long-term fertilizer treatments, we sampled nine bacterial co-occurrence communities and compared the difference between interspecies resource consumption patterns and network structure. Despite the differences between fertilizer treatments, the negative correlation ratios of interaction networks in soil aggregates were macroaggregates > microaggregates > silt + clays. Likewise, NPK-supplement (chemical fertilizer) had also decreased the number of positive correlations of the interaction network than M-supplement (organic fertilizer), regardless of the size of soil aggregates. Linear model analysis revealed that interspecies trophic patterns, including niche overlap and nestedness, drove bacterial competition in the interaction networks. Most importantly, interspecies niche overlap may be the intrinsic factor in the effects of fertilizer treatments and soil aggregates on bacterial interactions. This study enhances our understanding of the potential for changes in species trophic patterns and might guide the promotion of land management. Despite that the influence of soil structure and fertilizer treatments on the bacterial community has been widely studied, how they drive interspecies interactions has not been largely explored. Connectance and nestedness were significantly correlated with bacterial interactions, but no differences were found in different soil aggregates and fertilizer treatments. However, interspecies niche overlap could respond to soil aggregates and fertilizer treatments and ultimately drive the bacterial interactions. This study enhances our understanding of the mechanism of microbial interactions and highlights the importance of trophic patterns in the bacterial community. Our findings extend knowledge for nutrient availability on interspecific interactions.
细菌间相互作用在应对人为干扰和土壤结构变化的生态功能中发挥着重要作用。然而，肥料施用方式和土壤颗粒大小如何驱动细菌间相互作用在很大程度上仍不清楚。为了评估长期施肥处理下土壤团聚体中的细菌相互作用模式，我们对9个细菌共现群落进行了采样，并比较了种间资源消耗模式和网络结构之间的差异。尽管施肥处理存在差异，但土壤团聚体中相互作用网络的负相关比例为大团聚体>微团聚体>粉砂+黏土。同样，无论土壤团聚体大小，与施用有机肥相比，施用化肥也减少了相互作用网络中的正相关数量。线性模型分析表明，包括生态位重叠和嵌套性在内的种间营养模式驱动了相互作用网络中的细菌竞争。最重要的是，种间生态位重叠可能是施肥处理和土壤团聚体对细菌相互作用产生影响的内在因素。本研究增进了我们对物种营养模式变化潜力的理解，并可能指导土地管理的优化。尽管土壤结构和施肥处理对细菌群落的影响已得到广泛研究，但它们如何驱动种间相互作用在很大程度上尚未得到充分探索。连通性和嵌套性与细菌相互作用显著相关，但在不同土壤团聚体和施肥处理中未发现差异。然而，种间生态位重叠能够响应土壤团聚体和施肥处理，并最终驱动细菌相互作用。本研究增进了我们对微生物相互作用机制的理解，并突出了营养模式在细菌群落中的重要性。我们的研究结果扩展了关于养分有效性对种间相互作用影响的知识。
