胰岛素作用的细胞内位点：线粒体三羧酸循环
Intracellular site of insulin action: mitochondrial Krebs cycle.
作者信息
Bessman S P, Mohan C, Zaidise I
出版信息
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5067-70. doi: 10.1073/pnas.83.14.5067.
Abstract
Effect of insulin on the oxidation of carbons-1,4 and -2,3 of succinate and their incorporation into protein were studied in isolated rat hepatocytes and diaphragm muscle pieces. Oxidation of carbons-2,3 of succinate and their incorporation into hepatocyte protein were stimulated significantly by insulin. Insulin had only a trivial effect on 14CO2 formation from the carboxyl carbons of succinate. These data suggest that insulin affects only those carbons of succinate that are metabolized in the intramitochondrial Krebs cycle.
摘要
在分离的大鼠肝细胞和膈肌肌片中研究了胰岛素对琥珀酸碳-1、4和碳-2、3的氧化及其掺入蛋白质的影响。胰岛素显著刺激了琥珀酸碳-2、3的氧化及其掺入肝细胞蛋白质的过程。胰岛素对琥珀酸羧基碳生成14CO2的影响微不足道。这些数据表明，胰岛素仅影响在线粒体内三羧酸循环中代谢的琥珀酸的那些碳原子。
相似文献
1
Intracellular site of insulin action: mitochondrial Krebs cycle.胰岛素作用的细胞内位点：线粒体三羧酸循环
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5067-70. doi: 10.1073/pnas.83.14.5067.
2
Ammonia inhibits insulin stimulation of the Krebs cycle: further insight into mechanism of hepatic coma.
Neurochem Res. 1991 Jul;16(7):805-11. doi: 10.1007/BF00965690.
3
Differential effects of insulin, epinephrine, and glucagon on rat hepatocyte mitochondrial activity.
Arch Biochem Biophys. 1991 May 15;287(1):18-23. doi: 10.1016/0003-9861(91)90382-s.
4
Amphibolic role of the Krebs cycle in the insulin-stimulated protein synthesis.
Arch Biochem Biophys. 1991 Aug 15;289(1):83-9. doi: 10.1016/0003-9861(91)90445-o.
5
Effect of insulin on the metabolic distribution of carbons 1, 2, and 3 of pyruvate.胰岛素对丙酮酸碳1、碳2和碳3代谢分布的影响。
Arch Biochem Biophys. 1986 Jul;248(1):190-9. doi: 10.1016/0003-9861(86)90416-9.
6
Impaired mitochondrial metabolism and reduced amphibolic Krebs cycle activity in diabetic rat hepatocytes.
Biochem Mol Biol Int. 1995 Dec;37(6):1079-89.
7
Extracellular phosphate requirement for insulin action on isolated rat hepatocytes.
Biochem Med Metab Biol. 1991 Jun;45(3):344-9. doi: 10.1016/0885-4505(91)90039-n.
8
Insulin as a probe of mitochondrial metabolism in situ.
Mol Cell Biochem. 1997 Sep;174(1-2):91-6.
9
Effect of adriamycin on electron transport in rat heart, liver, and tumor mitochondria.阿霉素对大鼠心脏、肝脏及肿瘤线粒体中电子传递的影响。
Exp Mol Pathol. 1987 Feb;46(1):123-35. doi: 10.1016/0014-4800(87)90036-0.
10
Oxidation of succinate and the control of the citric acid cycle in the mitochondria of guinea-pig liver, mammary gland and kidney.豚鼠肝脏、乳腺和肾脏线粒体中琥珀酸的氧化与柠檬酸循环的调控
Biochem J. 1963 Jun;87(3):639-48. doi: 10.1042/bj0870639.
引用本文的文献
1
Therapeutic Effects of Neuro-Cells on Amyloid Pathology, BDNF Levels, and Insulin Signalling in APPswe/PSd1E9 Mice.神经细胞对APPswe/PSd1E9小鼠淀粉样病理、脑源性神经营养因子水平及胰岛素信号传导的治疗作用
Cells. 2025 Aug 20;14(16):1293. doi: 10.3390/cells14161293.
2
Insulin Diminishes Superoxide Increase in Cytosol and Mitochondria of Cultured Cortical Neurons Treated with Toxic Glutamate.胰岛素可减少毒性谷氨酸处理培养皮质神经元胞质和线粒体中超氧化物的增加。
Int J Mol Sci. 2022 Oct 20;23(20):12593. doi: 10.3390/ijms232012593.
3
Brain Insulin Resistance: Focus on Insulin Receptor-Mitochondria Interactions.脑胰岛素抵抗：聚焦胰岛素受体与线粒体的相互作用。
Life (Basel). 2021 Mar 22;11(3):262. doi: 10.3390/life11030262.
4
Insulin receptor in the brain: Mechanisms of activation and the role in the CNS pathology and treatment.脑内胰岛素受体：激活机制及其在中枢神经系统疾病和治疗中的作用。
CNS Neurosci Ther. 2018 Sep;24(9):763-774. doi: 10.1111/cns.12866. Epub 2018 Apr 24.
5
From in vivo to in vitro: Major metabolic alterations take place in hepatocytes during and following isolation.从体内到体外：在肝细胞分离过程中及分离后会发生主要的代谢改变。
PLoS One. 2017 Dec 28;12(12):e0190366. doi: 10.1371/journal.pone.0190366. eCollection 2017.
6
Interaction between plasma fetuin-A and free fatty acids predicts changes in insulin sensitivity in response to long-term exercise.血浆胎球蛋白-A与游离脂肪酸之间的相互作用可预测长期运动后胰岛素敏感性的变化。
Physiol Rep. 2017 Mar;5(5). doi: 10.14814/phy2.13183.
7
H2O2 Signalling Pathway: A Possible Bridge between Insulin Receptor and Mitochondria.H2O2 信号通路：胰岛素受体与线粒体之间的可能桥梁。
Curr Neuropharmacol. 2012 Dec;10(4):311-20. doi: 10.2174/157015912804143559.
8
Mitochondrial respiratory chain is involved in insulin-stimulated hydrogen peroxide production and plays an integral role in insulin receptor autophosphorylation in neurons.线粒体呼吸链参与胰岛素刺激的过氧化氢生成，并在神经元中胰岛素受体自身磷酸化过程中发挥不可或缺的作用。
BMC Neurosci. 2007 Oct 8;8:84. doi: 10.1186/1471-2202-8-84.
9
Hexokinase binding to mitochondria: a basis for proliferative energy metabolism.己糖激酶与线粒体的结合：增殖性能量代谢的基础。
J Bioenerg Biomembr. 1997 Aug;29(4):331-8. doi: 10.1023/a:1022442629543.
10
Insulin as a probe of mitochondrial metabolism in situ.
Mol Cell Biochem. 1997 Sep;174(1-2):91-6.
本文引用的文献
1
The effect of insulin on oxidations in isolated muscle tissue.胰岛素对离体肌肉组织氧化作用的影响。
Biochem J. 1938 May;32(5):913-25. doi: 10.1042/bj0320913.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Diabetes mellitus: observations, theoretical and practical.糖尿病：观察、理论与实践
J Pediatr. 1960 Feb;56:191-203. doi: 10.1016/s0022-3476(60)80119-9.
4
Glucose penetration into liver.葡萄糖渗透入肝脏。
Am J Physiol. 1958 Mar;192(3):491-6. doi: 10.1152/ajplegacy.1958.192.3.491.
5
In vitro protein degradation measured by differential loss of labeled methionine and 3-methylhistidine: The effect of Insulin.
Anal Biochem. 1981 Nov 15;118(1):17-22. doi: 10.1016/0003-2697(81)90150-0.
6
Insulin "inhibition" of gluconeogenesis by stimulation of protein synthesis.
Biochem Med. 1981 Dec;26(3):403-26. doi: 10.1016/0006-2944(81)90016-8.
7
Compartmentation of hexokinase and creatine phosphokinase, cellular regulation, and insulin action.己糖激酶和肌酸磷酸激酶的区室化、细胞调节及胰岛素作用。
Curr Top Cell Regul. 1980;16:55-86. doi: 10.1016/b978-0-12-152816-4.50007-8.
8
A molecular basis for the mechanism of insulin action.
Am J Med. 1966 May;40(5):740-9. doi: 10.1016/0002-9343(66)90155-0.
9
Hexokinase acceptor theory of insulin action. New evidence.
Isr J Med Sci. 1972 Mar;8(3):344-52.
Zotero 插件