Meles Dewa Ketut, Rachmawati Kadek, Hamid Iwan Sahrial, Mustofa Imam, Wurlina Wurlina, Suwasanti Niluh, Putri Desak Ketut Sekar Cempaka, Utama Suzanita
Laboratory of Veterinary Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Kampus C Mulyorejo, Surabaya 60115, Indonesia.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Kampus C Mulyorejo, Surabaya 60115, Indonesia.
Vet Med Int. 2022 Feb 21;2022:3685686. doi: 10.1155/2022/3685686. eCollection 2022.
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse effects of dioxin pollution exposure are needed in all aspects, including reproductive health. This study aimed to determine the effect of -tocopherol on superoxide dismutase (SOD) and malondialdehyde (MDA) levels, live spermatozoa, apoptosis, and necrosis in male rats exposed to dioxin as a model. Thirty healthy 12-week-old male rats were randomly divided into five groups. Rats in the control group were given corn oil twice daily at 4-hour intervals. The remaining rats were given TCDD 700 mg/kg BW daily, followed by administration of corn oil and -tocopherol at doses of 77, 140, and 259 mg/kg BW/d for T0, T1, T2, and T3 groups, respectively. The treatments were conducted for 45 days; all rats were euthanized to collect blood and testicular samples on day 46. The results showed that exposure of TCDD resulted in a decrease in SOD activity and live spermatozoa and increased MDA level and death, apoptosis, and necrosis of spermatozoa (T0) compared to the control (C) group ( < 0.05). The administration of -tocopherol, starting from the doses of 77 (T1), 149 (T2), and 259 mg (T3) per kg BW, was sequentially followed by returning MDA levels, recovering SOD activities, and restoration in the percentage of living, dead, apoptotic, and necrotic spermatozoa, similar ( > 0.05) to those of the control group. It could be concluded that the administration of -tocopherol resolves the harmful effects of TCDD on the viability of spermatozoa in rats as a model.
2,3,7,8-四氯二苯并-对-二噁英（TCDD）是一种持久性有机污染物，可诱导活性氧（ROS）过量产生。在包括生殖健康在内的各个方面都需要开展研究以避免二噁英污染暴露的不良影响。本研究旨在以二噁英暴露的雄性大鼠为模型，确定α-生育酚对超氧化物歧化酶（SOD）和丙二醛（MDA）水平、活精子、细胞凋亡及坏死的影响。将30只健康的12周龄雄性大鼠随机分为五组。对照组大鼠每隔4小时每日给予两次玉米油。其余大鼠每日给予700 mg/kg体重的TCDD，随后T0、T1、T2和T3组分别给予剂量为77、140和259 mg/kg体重/天的玉米油和α-生育酚。处理持续45天；在第46天对所有大鼠实施安乐死以采集血液和睾丸样本。结果显示，与对照组（C）相比，TCDD暴露导致SOD活性和活精子数量减少，MDA水平升高，精子死亡、凋亡及坏死增加（T0组，P < 0.05）。从每千克体重77 mg（T1组）、149 mg（T2组）和259 mg（T3组）的剂量开始给予α-生育酚后，依次出现MDA水平恢复、SOD活性恢复，以及活精子、死精子、凋亡精子和坏死精子百分比恢复，与对照组相似（P > 0.05）。可以得出结论，给予α-生育酚可消除TCDD对作为模型的大鼠精子活力的有害影响。
