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Role of reactive oxygen species in male infertility: An updated review of literature.活性氧在男性不育中的作用：文献综述更新
Arab J Urol. 2017 Dec 8;16(1):35-43. doi: 10.1016/j.aju.2017.11.001. eCollection 2018 Mar.
2
Technical Note: A new device for cervical insemination of sheep - design and field test.技术说明：一种绵羊宫颈内人工授精的新装置——设计与田间试验。
J Anim Sci. 2017 Dec;95(12):5263-5269. doi: 10.2527/jas2017.1951.
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Effect of l-arginine addition on long-term storability of ram semen.添加L-精氨酸对公羊精液长期保存性能的影响。
Andrologia. 2017 Dec 28. doi: 10.1111/and.12945.
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Dietary l-arginine supplementation improves semen quality and libido of boars under high ambient temperature.日粮补充 l-精氨酸可改善高温环境下公猪的精液质量和性欲。
Animal. 2018 Aug;12(8):1611-1620. doi: 10.1017/S1751731117003147. Epub 2017 Dec 4.
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Computational flow cytometry reveals that cryopreservation induces spermptosis but subpopulations of spermatozoa may experience capacitation-like changes.计算流式细胞术显示，冷冻保存会诱导精子凋亡，但精子亚群可能会经历类似获能的变化。
Reproduction. 2017 Mar;153(3):293-304. doi: 10.1530/REP-16-0539. Epub 2016 Dec 13.
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Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm.冷冻保存对牛精子活力、活性氧合成及DNA损伤的影响。
Theriogenology. 2016 Jul 15;86(2):562-71. doi: 10.1016/j.theriogenology.2016.02.007. Epub 2016 Feb 17.
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Molecular evidence of apoptotic pathway activation in semen samples with high DNA fragmentation.DNA碎片化程度高的精液样本中凋亡途径激活的分子证据。
In Vivo. 2015 Mar-Apr;29(2):289-94.
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Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development.精子凋亡的原因与后果；对不育症的影响及对发育的作用。
Int J Dev Biol. 2013;57(2-4):265-72. doi: 10.1387/ijdb.130146ja.
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The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen.牛血清白蛋白和胎牛血清对液态贮存兔精液精子质量、DNA 碎片化和脂质过氧化的影响。
Cryobiology. 2013 Aug;67(1):1-6. doi: 10.1016/j.cryobiol.2013.04.002. Epub 2013 Apr 15.
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The hypo-osmotic swelling test for evaluation of sperm membrane integrity.用于评估精子膜完整性的低渗肿胀试验。
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在脱脂乳稀释液中添加L-精氨酸可在冷藏温度下维持山羊精子质量达五天。

Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days.

作者信息

Susilowati Suherni, Triana Indah Norma, Wurlina Wurlina, Arimbi Arimbi, Srianto Pudji, Mustofa Imam

机构信息

Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Jl. Mulyorejo, Surabaya - 60115, Surabaya, Indonesia.

Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Jl. Mulyorejo, Surabaya - 60115, Surabaya, Indonesia.

出版信息

Vet World. 2019 Nov;12(11):1784-1789. doi: 10.14202/vetworld.2019.1784-1789. Epub 2019 Nov 15.

DOI:10.14202/vetworld.2019.1784-1789
PMID:32009757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6925050/
Abstract

AIM

The purpose of this study was to determine the benefits of L-arginine addition in skim milk extender to maintain the quality of goat spermatozoa in chilled storage.

MATERIALS AND METHODS

A total of 18 ejaculates from three healthy goats with weight and age of 45 kg and 4-5 years, respectively, were divided into three groups. The control group contained goat semen diluted in a skim milk extender without L-arginine; Treatment I and Treatment II contained goat semen diluted in a skim milk extender with added L-arginine 4 and 6 mM, respectively. These three groups were chilled at 5°C and evaluated daily for 5 days. Observed variables were viability, motility, intact plasma membrane (IPM), malondialdehyde (MDA) level, necrosis, and apoptosis of spermatozoa.

RESULTS

The addition of L-arginine 4 mM was the best treatment in maintaining viability, motility, and IPM and a decreased MDA level, percentage of necrosis, and apoptosis of goat spermatozoa. An ejaculate in this extender can be divided into 37 doses for intracervical insemination in <1 ml volume with 125 million motile spermatozoa.

CONCLUSION

Goat semen retained its quality when kept for 5 days in chilled storage by adding L-arginine in skim milk extender.

摘要

目的

本研究旨在确定在脱脂乳稀释液中添加L-精氨酸对冷藏保存山羊精子质量的益处。

材料与方法

从三只体重45千克、年龄4至5岁的健康山羊采集的共计18份精液样本被分为三组。对照组包含用不含L-精氨酸的脱脂乳稀释液稀释的山羊精液；处理组I和处理组II分别包含用添加了4 mM和6 mM L-精氨酸的脱脂乳稀释液稀释的山羊精液。这三组在5°C下冷藏，并连续5天每日进行评估。观察的变量包括精子的活力、运动能力、完整质膜（IPM）、丙二醛（MDA）水平、坏死情况和凋亡情况。

结果

添加4 mM L-精氨酸是维持山羊精子活力、运动能力和完整质膜以及降低MDA水平、坏死百分比和凋亡率的最佳处理方式。用这种稀释液稀释的一份精液可分为37剂，用于子宫颈内授精，每份体积小于1毫升，含1.25亿条活动精子。

结论

通过在脱脂乳稀释液中添加L-精氨酸，山羊精液在冷藏保存5天时能保持其质量。