Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau, Institut d'Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, Madrid, Spain.
Methods Mol Biol. 2022;2419:283-292. doi: 10.1007/978-1-0716-1924-7_17.
This chapter provides details on a simple and reproducible method used to determine the capacity of murine HDL to prevent the oxidation of LDL . The principle of the method is based on the rearrangement of double bonds of polyunsaturated fatty acids that occurs during the oxidation of human LDL , which generates a sigmoidal curve. The shape and length of the curve is modified in the presence of HDL , and such modifications are easily quantifiable by measuring the absorbance of conjugated dienes at 234 nm. The general technique described herein may be applied to evaluate the effect of HDL obtained from different experimental murine models of atherosclerosis.
本章提供了一种简单且可重复的方法，用于测定鼠 HDL 预防 LDL 氧化的能力。该方法的原理基于人 LDL 氧化过程中多不饱和脂肪酸双键重排，生成 S 形曲线。在 HDL 的存在下，曲线的形状和长度发生改变，通过测量 234nm 处共轭二烯的吸光度可以很容易地对这种改变进行定量。本文所述的一般技术可用于评估从不同动脉粥样硬化实验性鼠模型中获得的 HDL 的效果。
